摘要
采用生物信息学工具及网络资源,对已在GenBank上注册的玉米、小麦、芝麻、菜豆、海茄冬、红三叶等植物的肌醇-1-磷酸合成酶的核酸及氨基酸序列进行分析,并对其组成成分、导肽、跨膜结构域、疏水性/亲水性、分子系统进化关系、蛋白二级及三级结构等进行预测及推断。结果表明:植物肌醇-1-磷酸合成酶在进化过程中非常保守,且可能为定位于细胞质的亲水性蛋白;结构预测表明不同来源的MIPS蛋白尽管在结构上有所差异,但是其催化位点及NAD+结合区基本一致,具有较强的保守性。
Using bioinformatics tools and resources from the Internet,the nucleic acid and amino acid sequences of myo-inositol-1-phosphate synthase from Zea Mays,Triticum aestivum,Sesamum indicum,Phaseolus vulgaris,Avicennia marina,Trifolium pretense,which were registered in GenBank,were analyzed.And the composition of amino acid sequences, transit peptides,transmembrane topological structures,hydrophobicity or hydrophilicity,molecular phylogenetic evolution,secondary and tertiary structure of protein were predicted and analyzed.The result shows that MIPS is relatively well-conserved throughout evolution,it's a hydrophilic protein located in the cytoplasm.The structure prediction indicates that the MIPS retains its the catalytic region and NAD+binding domain intact throughout the evolution of organisms despite allowing diversification elsewhere in its structure.
出处
《生物技术通报》
CAS
CSCD
2008年第S1期366-373,共8页
Biotechnology Bulletin
基金
国家自然科学基金(NO.30570178)
