期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

小鼠胚胎玻璃化冷冻研究 被引量：1

A study on vitrification of mouse embryo
下载PDF
导出
摘要 目的:观察不同温度对小鼠胚胎玻璃化冷冻的影响,探索小鼠胚胎玻璃化冷冻的最适宜温度。方法:选用8周龄昆明系小鼠超排卵,取桑葚胚或早期囊胚在不同温度下玻璃化冷冻,解冻后体外培养,观察其48小时发育率和孵化率。结果:25℃时胚胎冷冻效果要优于4℃(P<0.01)及37℃时(P<0.05)。25℃时和对照组之间无差异。结论:温度对小鼠胚胎玻璃化冷冻保存有影响,25℃为最适温度。 [Objective]By an observation of the development of the thawed mouse embryos,to find the most suitable temperature of vitrification.[Method]Mouse monilae or blastocyst from in vivo were frozen by vitrification at 4℃,25℃and 37℃respectively,then the developmental ability of the thawed mouse embryos was assessed.[Result]the hatch rate of thawed mouse embryos vitrified at 25℃is higher than that at 4℃or 37℃.[Conclusion]Temperature is ah important element for the vitrification of mouse embryo,and the 25℃is the most suitable temperature.
作者 邹立波 费小阳 王丽卿
机构地区 金华市人民医院
出处 《浙江医学教育》 2006年第2期38-39,42,共3页 Zhejiang Medical Education
基金 浙江省卫生厅课题(编号2004A087)资助
关键词 乙二醇 小鼠胚胎 玻璃化冷冻 ethylene glycol mouse embryo vitriiication
分类号 R321-33 [医药卫生—人体解剖和组织胚胎学]
  • 相关文献

参考文献6

  • 1Gordts S,Roziers P,Campor R et al.Survival and pregnancy outcome after ultrarapid freezing of human embryos[].Fertility and Sterility.1990
  • 2Fahy G.M,MacFarlane,D.R,Angell,C.A.et al.Vitrification as an approach to cryopreaervation[].Cryobiology.
  • 3Whittingham D G,Leibo S P,Mazur P.Survival of mouse embryos frozen to -196℃ and -269℃[].Science.1972
  • 4Trounson A,Mohr L.Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo[].Nature.1983
  • 5Rall WF,Fahy GM.Ice-free cryopreservation of mouse embryos at -196°C by vitrification[].Nature.1985
  • 6Kasai M.Simple and efficient methods for vitrification of mammalian embryos[].Animal Reproduction Science.1996

同被引文献19

  • 1宋文妍,孙莹璞,金海霞.不同冷冻方法对小鼠不同成熟时期卵母细胞纺锤体的影响[J].现代妇产科进展,2007,16(5):362-365.
  • 2黄国宁,孙海翔.体外受精-胚胎移植实验室技术[M].北京:人民卫生出版社,2012:244.
  • 3Staehecki J,Cohen J. An overview of oocyte cryopreserva- tion [J]. Reproductive Biomedicine Online,2004,9 (2) : 152-163.
  • 4Prentice JR,Anzar M. Crypreservation of mammalian oocyte for conservation of animal genetics [J]. Vet Med Int, 2009, 2011:11.
  • 5Monzo C, Haouzi D, Roman K, et al. Sloe freezing and vit- rification differentially modify the gene expression profile of human metaphaseH oocyte [J]. Hum Reprod, 2012, 27 (7) :2160-2168.
  • 6Chamayou S, Bonaventura G, Alecei C, et al. Consequences of metaphase lI oocyte cryopreservation on mRNA con- tent [J]. Cryobiology,2011,62(2) : 130-134.
  • 7Noyes N, Boldt J,Nagy ZP. Oocyte cryopreservation :is it time to remove its experimental label ? [J]. J Assist Reprod Genet,2010,27(5) :69-74.
  • 8Forman E J, Li X, Ferry KM, et al. Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after in- tracytoplasmic sperm injection : a novel, paired randomized controlled trial using DNA fingerprinting [J]. Fertil Steril, 2012,98(3) :644-649.
  • 9Wininger JD,Kort HI.Cryopreservation of immature and ma- ture human oocytes [J]. Seminars Reprod Medi,2002,20 ( 1 ) : 45-49.
  • 10Rail W, Fahy G. Ice-free cryopreservation of mouse em- bryos at-196 degrees C by vitrification [J]. Nature, 1985 (6003) : 573-575.

引证文献1

浙江医学教育
2006年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部