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Determination of free energy of protein folding on liquid-solid interface 被引量:5

Determination of free energy of protein folding on liquid-solid interface
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摘要 Based on the fact that the stoichiometric displacement model for retention of solute and the total adsorption free energy of solute on a solid surface can be divided into two components, net adsorption and net desorbed energies, a new principle and an equation for calculating the free energy of protein folding, △△GF, on the solid surface are proposed. With high-performance hydrophobic interaction chromatography (HPHIC), an experimental method for determining the △△GF is established. Lysozyme and a-amylase have been selected as examples to test the new method, and their △△GF on the HPHIC stationary phase surface are found to be much higher than that reported from a solution. In addition, the △△GF of the two proteins are found to increase with the concentration of denaturing agent employed. The average standard deviations,±4.7% for lysozyme and ± 3.0% for a-amylase, indicate that the new method has a satisfactory reproducibility and reliability. Based on the fact that the stoichiometric displacement model for retention of solute and the total adsorption free energy of solute on a solid surface can be divided into two components, net adsorption and net desorbed energies, a new principle and an equation for calculating the free energy of protein folding, ΔΔGF, on the solid surface are proposed. With high-performance hydrophobic interaction chromatography (HPHIC), an experimental method for determining the ΔΔGF is established. Lysozyme and α-amylase have been selected as examples to test the new method, and their ΔΔGF on the HPHIC stationary phase surface are found to be much higher than that reported from a solution. In addition, the ΔΔGF of the two proteins are found to increase with the concentration of denaturing agent employed. The average standard deviations, ±4.7% for lysozyme and ±3.0% for α-amylase, indicate that the new method has a satisfactory reproducibility and reliability.
机构地区 NW Univ Xian
出处 《Chinese Science Bulletin》 SCIE EI CAS 2000年第3期237-241,共5页
关键词 STOICHIOMETRIC displacement model high-performance HYDROPHOBIC interaction CHROMATOGRAPHY adsorption free energy protein folding. stoichiometric displacement model high-performance hydrophobic interaction chromatography adsorption free energy protein folding
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参考文献3

  • 1J. Chang,L. Guo,W. Feng,X. Geng.Studies on the correlation of chromatographic behaviour of biopolymers between reversed-phase and hydrophobic interaction chromatography. Testing the stoichiometric displacement between biopolymer and displacing agent[J].Chromatographia (-).1992(11-12)
  • 2Ptisyn,O. B.A determinable but unresolved problem[].The FASEB Journal.1996
  • 3Anfinsen,C.B.Principles that govern the folding of protein chains[].Science.1973

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