摘要
For abundant expression of antisense RNA, triplex forming RNA and Ribozyme in vivo, a novel vector pBSKneo rU6’ was constructed by PCR cloning. This vector contains the intact human snRNA U6 gene expression unit, yet replacing the 61 nt sequence in the middle of U6 snRNA coding region with three restriction enzyme sites. Hela nuclear extract in vitro transcription experiments demonstrated that this vector can effectively express U6 mutant RNA. Containing neo r at the same time, stably transfected pBSKneo rU6’ can be selected easily.
For abundant expression of antisense RNA, triplex forming RNA and Ribozyme in vivo, a novel vector pBSKneo rU6' was constructed by PCR cloning. This vector contains the intact human snRNA U6 gene expression unit, yet replacing the 61 nt sequence in the middle of U6 snRNA coding region with three restriction enzyme sites. Hela nuclear extract in vitro transcription experiments demonstrated that this vector can effectively express U6 mutant RNA. Containing neo r at the same time, stably transfected pBSKneo rU6' can be selected easily.
基金
ProjectsupportedbytheGrantNo .G19980 5 110 3fromStateKeyProgramsBasicResearchofChinaandGrantNo .KJ95 1 B1 610fromChi neseAcademy
