摘要
通过对分离自洗煤厂的一株青霉菌进行人工诱变，选育出一株具有较强降解褐煤能力的突变株．将褐煤加到平板培养的该菌株上，经过３６ ｈ 开始被降解．将该菌株用摇瓶培养３ ｄ ，其培养液也能降解褐煤．元素分析结果表明，降解物中 Ｈ、 Ｏ、 Ｎ 的元素质量分数（ ｗ／ ％ ） 比原煤均有所升高，而ｗ（ Ｃ）／ ％ 明显下降．降解产物的经验分子式可写为 Ｃ１００ Ｈ１４４ Ｏ５８ ．２ Ｎ１０ ．０ Ｓ１ ．１８ ，经凝胶过滤法测，定其 Ｍｒ 分布在（２ ～８） ×１０４ 之间； 其红外吸收光谱与原煤相比也有一定的变化．
A strong lignite degrading mutant was selected by UV treatment of Penicillium sp. which was isolated from coalmine slurry. The mutant could start to degrade lignite from 36 h after adding lignite onto the mycellium incubated in plate, and the filtrate of the liquid culture incubated for 3 d in shaking condition could also degrade lignite. Elemental analysis indicated that compared with the original lignite, the composition of the degraded products changed. The percents of H, O, and N contents ( w /%) were increased, but the percent w (C)/% content was decreased. According to the principle of the simplest molecular formula, the molecular formula of the bioproduct could be written as C 100 H 144 O 58.2 N 10.0 S 1.18 . The M r of the bioproduct was estimated between (2～8)×10 4. Its IR spectra were also different from the original lignite.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第S1期21-24,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金
关键词
真菌
降解
褐煤
fungi
degradation
lignite
