摘要
根据已知的甘薯块根贮藏蛋白基因序列, 设计和合成了两对PCR引物。用PCR方法,从南薯88基因组中分别扩增出两种DNA片段: 一种含有贮藏蛋白基因启动子和核糖体结合序列(SP2);第二种不仅含有第一种DNA片段的全部序列,而且还有贮藏蛋白的信号肽编码序列(SP1)。核苷酸序列分析表明,这两种DNA片段的启动子和核糖体结合区虽然具有很高的同源性(82% ), 但它们并不相同。在SP1和SP2 的基因启动子区都存在几种典型的保守序列, 如受糖诱导调控的蔗糖盒(Suc-box),CAAT盒和TATA盒。用这两种DNA片段分别构建了可调控的甘薯高效表达载体。
Two pairs of primers were synthesized on the basis of known promoter sequences of sweet potato storage protein (sporamin) and two kinds of DNA fragments were obtained by using polymerase chain reaction from Ipomoea batatas cv.Nanshu 88 genome. One of the fragments (called SP2) contains both sequences of promoter and ribosome binding site (RBS),while the another fragment (called SP1) contains not only promoter and RBS but also the signal peptide coding sequence. DNA sequence analysis showed that SP1 and SP2 had high sequence homology (82%) in the promoter and RBS region. There are several conserved sequences in promoter region of SP1 and SP2, such as sucrose box,CAAT box and TATA box.Two specific expression vectors were constructed by using these two fragments for foreign genes expression in sweet potato.
出处
《西南农业学报》
CSCD
北大核心
1999年第S2期116-120,共5页
Southwest China Journal of Agricultural Sciences
基金
四川省 "九五"重点科学技术研究资助
关键词
甘薯
贮藏蛋白基因
基因启动子
序列测定
同源分析
表达载体
Ipomoea batatas
sporamin gene
gene promoter
sequencing
homology analysis
expression vector
