摘要
利用短期诱导实验,最大程度消除诱导子以外其它因素的影响。利用刺激响应机制,研究了在硝酸银、花生四烯酸短期诱导下,紫杉醇生物合成途径中关键代谢产物的质量浓度变化模式。实验表明,在10μmol/L硝酸银诱导下,Baccatin Ⅲ,10-DAB(10-deactyle-Baccatin Ⅲ)的质量浓度出现下降,同时紫杉醇的质量浓度提高到对照组的三倍,三种物质质量浓度变化呈现负相关;在0.1μmol/L花生四烯酸诱导下,Baccatin Ⅲ,10-DAB出现上升,同时紫杉醇质量浓度也比对照组高出三倍,三种物质质量浓度变化呈现正相关。诱导作用下的中间代谢产物的变化模式为从次生代谢水平对诱导子进行分类提供了依据,提供了诱导子对紫杉醇生物合成途径的影响信息与配伍信息。
The short time elicitation experiments, which could eliminate the effect of other factors in process, were carried out. The concentration change modes of key metabolites in taxol biosynthesis pathway had been got with silver nitrate and arachidonic acid elicitation respectively. The result showed that silver nitrate at the concentration of 10 μmol/L could improved the production of taxol to 3-fold than that in control experiment and reduce the concentration of 10-DAB and Baccatin Ⅲ. Meanwhile, arachidonic acid at the concentration of 0.1 μmol/L could improve the production of taxol to 4-fold than that in control experiment and raise the concentration of 10-DAB and Baccatin Ⅲ simultaneously. The concentration change model for metabolite during induction could be used to classify elicitor at secondary metabolism level. Moreover, it could reveal the effect of elicitor on the taxol biological biosynthesis pathway and provide the information in elicitor cooperation.
出处
《食品与生物技术学报》
CAS
CSCD
1999年第5期35-38,共4页
Journal of Food Science and Biotechnology
基金
教育部"跨世纪优秀人才培养计划"基金
天津市重点攻关项目
