摘要
用化学合成的方法合成了水蛭素12肽基因的编码序列,通过DNA重组技术将水蛭素12肽基因片段与低分子量单链尿激酶cDNA片段连接构建了融合基因。融合基因在大肠杆菌中获得表达。体外实验结果表明,表达的融合蛋白具有溶纤活性和抗凝活性。
A hybrid protein was constructed by fusion of the 12aa of the COOH-terminal of hirudin to the NH2-terminal of low molecular weight single-chain urokinase -type plasminogen activator. We have expressed the hybrid protein in Eschericliia colt and proved it has both fibrinolytic activity and anticoagulant activity.
出处
《生物技术通讯》
CAS
1996年第3期114-117,共4页
Letters in Biotechnology
基金
国家自然科学基金
关键词
水蛭素12肽
低分子量单链尿激酶
溶纤活性
抗凝活性
Peptide 12 derived from Hirudin Low molecular weight single-chain urokinase Fibrinolytic activity Anticoagulant activity
