摘要
本实验以~3H-TdR(~3H-胸腺嘧啶核苷)掺入法研究了相同碳链长度的硒化不饱和脂酸及相应未硒化脂酸对7402人肝癌细胞株DNA合成的抑制作用。实验证明,终浓度为150ug/ml的受试物对人肝癌细胞株DNA合成的抑制作用以硒化亚麻酸量强,与硒化亚油酸、硒化油酸和油酸相比,差异非常显著(P【0.01),与亚油酸相比,差异显著(P【0.05),但与亚麻酸相比,差异未达统计学的显著程度(P】0.05),硒化亚油酸与亚油酸之间无差异(P】0.05),而硒化油酸与油酸则差异非常显著(P【0.01)。表明:含2-3个双键的脂酸,硒化与否,抑制癌细胞DNA合成强度差异不大,但对仅含一个双键者,硒化可显著增强对DNA合成的抑制作用。各种受试物对人肝癌细胞株的杀伤作用也以硒化亚麻酸最强。
The inhibition of unsaturatecl fatty acids and their serenides on DNA synthesis in human hepatocarcinoma cell line 7402 was studied by the 3H- TdR incorporation. It was demonstrated that selenide of linolenic acid was the most strong inhibition of DNA synthesis among these fatty acids,when the concentration was 150 μg/ml. It was stronger than linoleic acid (P <0. 05)、oleic acid (P<0. 01)、selenide of linoleic acid and oleic acid (P<0. 01). But there was no remarkable difference in inhibition between linolenic acid and its selenide and between linoleic acid and its selenide (P>0. 05). There was remarkable difference between oleic acid and its selenide (P<0. 01). It was demonstrated that there was no difference whether fatty acids with 2 or 3 double bonds Contain selenium or not. And it can enhance the inhibition of DNA synthesis,if the fatty acids with only one bond containing selenium.
出处
《广州医学院学报》
1995年第2期5-9,共5页
Academic Journal of Guangzhou Medical College
关键词
硒化不饱和脂酸
不饱和脂酸
人肝癌细胞株
DNA合成抑制
Selenide of unsaturated fatty acid
unsaturated fatty acid
inhibition of DNA synthesis
human hepatocarinoma cell line.
