摘要
香荚兰幼茎在ＭＳ＋ＢＡ１＋ＮＡＡ５培养基上培养４０ｄ，其表面形成白色、团状的愈伤组织，这类愈伤组织可继代培养而增殖。愈伤组织在ＭＳ＋ＢＡ２－３＋ＮＡＡ（０．５）培养基上培养３０ｄ后形成许多颗粒状原球茎，原球茎可进一步继代增殖。将原球茎接种在１／２ＭＳ＋ＢＡ１＋ＩＢＡ（０．５）培养基上培养约３０ｄ，原球茎即可发育形成小植株，小植株进一步增殖形成丛生芽。无根苗在１／２ＭＳ＋１ＡＡ１培养基上培养２０ｄ后生根，移栽成活率达１００％。
Young stems of Vanilla planifolia were cultured on Ms medium containing 1.0μg/mL BAand 5.0
μg/mL NAA,and white calli occurred in 40 days,Such calli could be subcultured manytimes for
propagation。The calli were transferred onto MS medium supplernented with 2-3 μg/mL BA
and 0.5μg/mL NAA,and a large numberof protocorms were induced in 30 days.Theprotocorms
subcultured on half strength MS medium containing 1.0μg/mL BA and 0.5 μg/mL IBA
produced many plantlets in 30 days,and multiple shoot buds were regenerated from
theplantlets.Rcots of the plantlets were induced in 20 days on half strength MS medium
containing1.0μg/mL IAA and a 100% success was attained in field establishment,
出处
《热带作物学报》
CSCD
1995年第S1期44-48,共5页
Chinese Journal of Tropical Crops
关键词
香荚兰
组织培养
愈伤组织
原球茎
再生植株
Vanilla planifolia Tissue culture Callus Protocorm Plant regeneration
