摘要
应用改良的Ｍａｒｔｉｎ（１９９３）定量反转录－聚合酶链式反应（ｒｅｖｅｒｓｅｔｒａｎｓｃｒｉｐｔｉｏｎ－ｐｏｌｙ－ｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＲＴ－ＰＣＲ）方法，测定了大鼠ＥＴＡ型受体（（Ｅｎｄｏｔｈｅｌｉｎ－Ａｒｅｃｅｐｔｏｒ，ＥＴＡ－Ｒ）ｍＲＮＡ在心血管系统细胞中的分布。结果证明，ＥＴＡ－ＲｍＲＮＡ广泛地分布在心血管系统细胞中．其中以平滑肌细胞中含量最高。内皮素及其受体的配基可以促进血管平滑肌细胞（ｖａｓｃｕｌａｒｓｍｏｏｔｈＭｕｓｃｌｅｃｅｌｌ，ＶＳＭＣ）中ＥＴＡ－ＲｍＲＮＡ的表达。此外，还发现在自发性高血压动物的血管平滑肌细胞中ＥＴＡ－Ｒ基因的高表达可能是高血压血管高反应性的一个重要机制。
With
Reverse Transcription- Polymerase Chain Reaction (RT-PCR ),expression
of endothelin- Areceptor(ETA - R) mRNA in the cells of rat
cardiovascular system was studied.It was found that(ETA- R) mRNA is
widely distributed in the cells of rat cardiovascular system.Among
them,(ETA- R)mRNA level is the highest in the smooth muscle
cells(SMCs) .Endothlin can significantly increase the(ETA - R) mRNA
level in SMC,and in addition,(ETA- R)mRNA levels are
significantly increased in spontaneously hypertensive rat VSMC,These
data show that the high expression of( ETA - R) gene in SMC may play
an important role in the pathogenesis of hypertension.
出处
《北京大学学报（医学版）》
CAS
CSCD
1994年第S1期29-32,共4页
Journal of Peking University:Health Sciences
关键词
内皮素A型受体
基因表达
高血压
Endothelin A receptor
Gene expression
Hypertension
