摘要
对担子菌ＬＭＰＱ３９菌丝原生质体进行了紫外光──ＬｉＣｌ复合处理后，得到突变株Ｕ３９，再将其分别用６０Ｃｏ、ＨＮＯ２两种方法处理，各获得突变株ＵＣｏ３９和ＵＮ３９，它们的ＣＭＣ酶活由原来ＬＭＰＱ３９的０．３１ｕ分别提高到０．４６ｕ和０．５７６ｕ．酯酶同功酶电泳表明，ＵＣｏ３９、ＵＮ３９和母株ＬＭＰＱ３９的酶谱出现了６条共同的谱带，同时也出现差异．突变株和母株的拮抗试验呈阳性。
In order to improve the CMCase activity of the Core Residue Cellulose-decomposing basidiomycetes LMPQ39, the author treated its protoplasts with a system of mutagenic agents such as UV-LiCL, 60Co and HNO2, resulting in two mutants UN39 and UCo39. Congo Red-Polysaccharide interaction was used in screening, and DNS assay system showed that the CMCase activity was improved from 0. 310u to 0. 576u and 0. 460u. Additionally,isoeasterase pattern and antagonism testified that the two mutants varied from the stool.
出处
《上海交通大学学报》
EI
CAS
CSCD
北大核心
1994年第S1期131-137,共7页
Journal of Shanghai Jiaotong University
关键词
担子菌
原生质体诱变
刚果红染色
CMC酶活
basidiomycetes, mutagenesis of protoplasts, Congo Red-Polysaccharide interaction, CMCase
