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细菌L型的组织印片法检测

TISSUE PRINT SLIDE TECHNIQUE IN THE DIAHNOSIS OF BACTERIAL L-FORMS
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摘要 应用组织印片革兰氏、细胞壁染色的方法,检查36例慢性扁桃体炎、15例慢性咽炎组织中的细菌L型。结果表明,组织印片革兰氏染色和细胞壁染色L型菌的检出率相同,阳性率是90.2%,与对照组中同一标本的L型培养和免疫组织化学染色的L型检出率均具有一致性(P>0.05)。上述4种检查方法的L型检出率之间亦无显著性差异(P>0.05)。故我们认为,组织印片法检测细菌L型简便、准确,是L型感染初步筛选的最佳方法,可用于L型感染的快速诊断。 Chronic inflammatory tissues due to bacterial L-forms were examined by tissue print slide technique with Gram's staining and cell walls staining (36 cases of chronic tonsillitis and 15 cases of chronic pharygitis). No difference was observed regarding the results obtained in both staining methods. The positive rate of bacterial L-forms was present in 90.2%. The results obtained by culture and immunohistochemical staining presented no significantly statisitical difference as compared with those mentioned above (P>0.05). It is suggested that tissue print slide technique is sipmle and reliable. It may be the technique of choice in screening infected tissues for bacterial L-forms, and may be employed prompt in the diagnosis of bacterial L-forms.
出处 《中国微生态学杂志》 CAS CSCD 1993年第1期56-59,共4页 Chinese Journal of Microecology
关键词 组织印片法 细菌L型 Tissue print slide technique Bacterial L-forms
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