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小鼠胚胎玻璃化冷冻保存影响因素的研究 被引量:3

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摘要 胚胎置玻璃化1液(VS-1)(含10%甘油+20%1.2一丙二醇+20%FCS的PBS液)中平衡10或17min后装管置4℃或18℃玻璃化2液(VS-2)(含25%甘油+25%1.2一丙二醇+20%FCS的PBS液)中。随即投入液氮冷冻保存。结果显示:在VS-1液中平衡10min组的胚胎获较高成活率;4℃的VS-2液显著地改善囊胚冷冻后成活率;桑椹胚冷冻效果优于囊胚。将解冻后120枚胚胎移植于12只受体鼠,获36只成活仔鼠。
出处 《中国比较医学杂志》 CAS 1993年第Z1期43-46,共4页 Chinese Journal of Comparative Medicine
基金 卫生部重点科技资助项目
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同被引文献30

  • 1鞠丽梅,三好一郎,笠井憲雪,蔡有余.显微注射法制备转基因小鼠的技术研究[J].中国实验动物学报,1999,7(1):11-15. 被引量:9
  • 2徐平.不同日龄和品系小鼠超排卵、体外受精及受孕率的比较研究[J].中国实验动物学杂志,2001,11(2):78-81. 被引量:16
  • 3李劲松,成国祥,李厚达.胚胎冷冻技术在转基因动物中的应用[J].生物技术,1996,6(5):1-4. 被引量:7
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