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Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B_4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits

Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B_4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits
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摘要 Modification was made on the reversed-phase high performance liquidchromatography(RP-HPLC)with Yue et al’s method as a base.The modified RP-HPLCwas used to detect leukotriene B<sub>4</sub>(LTB<sub>4</sub>)and 5-hydroxyeicosatetraenoic acid(5-HETE).Itwas found that the modified method has the merits of simpler procedures,shortertesting time and more satisfactory efficacy. Modification was made on the reversed-phase high performance liquid chromatography(RP-HPLC)with Yue et al's method as a base.The modified RP-HPLC was used to detect leukotriene B_4(LTB_4)and 5-hydroxyeicosatetraenoic acid(5-HETE).It was found that the modified method has the merits of simpler procedures,shorter testing time and more satisfactory efficacy.
出处 《Journal of Medical Colleges of PLA(China)》 CAS 1993年第2期192-196,共5页 中国人民解放军军医大学学报(英文版)
关键词 LEUKOTRIENE B<sub>4</sub> 5-hydroxyeicosatetraenoic acid chromatography high performance liquid NEUTROPHILS animal RABBITS leukotriene B_4 5-hydroxyeicosatetraenoic acid chromatography high performance liquid neutrophils animal rabbits
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