摘要
Modification was made on the reversed-phase high performance liquidchromatography(RP-HPLC)with Yue et al’s method as a base.The modified RP-HPLCwas used to detect leukotriene B<sub>4</sub>(LTB<sub>4</sub>)and 5-hydroxyeicosatetraenoic acid(5-HETE).Itwas found that the modified method has the merits of simpler procedures,shortertesting time and more satisfactory efficacy.
Modification was made on the reversed-phase high performance liquid chromatography(RP-HPLC)with Yue et al's method as a base.The modified RP-HPLC was used to detect leukotriene B_4(LTB_4)and 5-hydroxyeicosatetraenoic acid(5-HETE).It was found that the modified method has the merits of simpler procedures,shorter testing time and more satisfactory efficacy.