摘要
本文采用二甲氨基偶氮苯(DAB)诱发的大鼠肝癌模型,运用组织病理学、血清学、免疫组化及免疫电镜技术相结合的方法,对诱癌过程中各个不同时期肝脏的组织病理学变化及肝癌阳性标志物AFP的表达进行了动态观察。结果显示:早在血清AFP浓度上升和由卵圆细胞转变而来的小肝细胞表达AFP之前,肝小叶中就出现了散在的AFP阳性肝细胞,我们认为这种AFP阳性肝细胞可作为肝细胞癌前病变的早期征象之一。在AFP阳性的肝细胞内,AFP主要定位于核周间隙、粗面内质网和高尔基复合体。
Histopathological changes as well as the expression of alpha-fetoprotein were studied during hepatoccarcinogenesisi induced by dimethylaminoazobenzene in rats by busing combined serological, histopathological, immunohistochemical and immunocytochemical techniques. The reslts indicated that some AFP-positive hepatocytes were seen scattered in lobules even before the rise of serum AFP level and before the expression of AFP in the transitional cells and small hepatocytes transformed from oval cells. It is considered that this kind of AFP-positive hepatocyte may be one of the early marks of precancerous changes of hepatocytes. In the AFP-positive hepatoeytes, the AFP was mainly localized in perinuclear spce (PNS), rough endoplasmic reticulum (RER) and Golgi complex.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1993年第3期250-255,共6页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家自然科学基金
关键词
肝癌发生
AFP免疫组化
免疫电镜
Hepatocarcinogenesis
AFP
Immunohistochemistry
Immunoelectron microscope
