摘要
本文采用体外培养技术,比较了4%、12%和20%体表面积Ⅲ度烧伤及其复合5Gyγ射线全身照射后小鼠骨髓造血微环境基质细胞及粗-单核系造血的变化。结果单烧(单纯烧伤)组早期造血主要表现为抑制作用,以大面积烧伤者严重。6天后则表现为刺激作用,以中等面积烧伤者明显。CFU-GM与成熟基质细胞的变化是平行的,且其两个数量高峰的出现与烧伤感染的两个高发期吻合。复合伤后早期,造血功能既低于单烧,也低于单照(单纯照射)组,后期则介于单烧和单照之间,实验结果未发现复合伤对造血的抑制有“大于两单烧之和”的现象。
In Vitro studies of bone marrow cells were performed to investigate the changes of mouse hemopoietic stromal cells and granupoiesis after third degree thermal burn of 4%, 12%and 20%body surface areas and their combined injuries with 500 rads total body y-irradiation. The thermal trauma exhibited hemopoietic suppression effect at early stage, especially in 20%-area group. But from day 6 post injury, it exhibited stimulating effect, with the 12%-area burn more significant. The change of marrow CFU-GM paralleled with that of mature stromal cell and its two peaks coincided with the two high incidence phases of burn-infection.Hemopoiesis was lower than those of both burns and radiation early after combined radiation-burn injury,while being between those of burn and radiation later. No synergistic phenomenon of combined damage greater than the sum of those by two single injuries was found in hemopoiesis studied in this experiment. radiation injury combined with thermal burn hematopoietic environment granulocytopoiesis bone marrow stromal cell CFU-GM CFU-F
出处
《中国实验血液学杂志》
CAS
CSCD
1993年第1期87-92,共6页
Journal of Experimental Hematology
