摘要
组织化学观察发现:大鼠硬脊膜外腔注入辣椒素后第7天,与溶媒对照组比较.脊髓后角Ⅰ一Ⅲ层内与一级感觉传入有关的神经递质P物质(SP)的免疫反应性(SP-IR)明显减弱,而与一级传入的调控有关的亮氨酸脑啡肽(ENK)的免疫反应性(ENK-IR)明显增强;同时后角Ⅱ层内及脊神经节A型细胞内一级传入C类纤维的标志酶抗氟化物酸性磷酸酶(FRAP)的反应产物消失.功能测定表明,应用辣椒素后,动物的痛阈明显升高,溶媒对痛阈无明显影响.由此提示:硬脊膜外腔注入辣椒素能有效地作用于一级传入C类纤维,影晌其递质水平、酶的活性及信息传导功能;这类辣椒素敏感的C类纤维与脊髓后角内含ENK的中间神经元之间可能存在着密切联系.
In this study histochemical method was used. It has been found that on the seventh day after epidural administration of capsaicin substance p like immuno- reactivity (SPAR) in laminae Ⅰ-Ⅱ of dorsal horn of spinal cord decreased obv- iously as compared with that of vehicle treated animals, meanwhile, the activity of fluoride resistant acid phosphatase (FRAP) in both laminae Ⅱ of dorsal horn of spinal cord and the B-type cells of spinal ganglion disappeared. On the cont- rary the Leu-enkephalin immunoreactivity (ENK-IR) in laminae Ⅰ-Ⅲ of dorsal horn of spinal cord apparently increased as compared with that in vehicle group. the evidence from functional measurement showed that capsaicin can raise pain threshold, however, vehicle didnot have obvious influence on pain threshold. The foregoing results suggest that epidural administration of capsaicin in adult rat can effect the level of neurontransmitters, the activity of enzymes and block the con- duction of the primary afferent C fibers; the capsaicin-sensitive C fibers may have relationship with the ENK-containing interneurons in dorsal horn of spinal cord.
出处
《解剖学报》
CAS
CSCD
北大核心
1991年第S1期55-60,142,共7页
Acta Anatomica Sinica
基金
国家自然科学基金
