摘要
酵母多糖刺激大鼠腹腔细胞合成与释放PGE_2及TXB_2,经短期培养(0～4h),静止的细胞合成的PGE_2虽随时间的延长而增加,但无显著差别,而TXB_2的增长有明显差别。经酵母多糖刺激后二者均有显著增加,但以TXB_2之增加更迅速。角叉菜、硅胶、钙离子载体A_(23187)均能显著促进培养24h的腹腔巨噬细胞合成与释放PGE_2和TXB_2。消炎痛、丹参素、硝苯吡啶及黄芩甙明显抑制A_(23187)诱导的PGE_2合成增加。
PGE2 has been recognized as an important mediator derived from macrophage in response to inflammatory stimulus. Among the cells involved in immune responses macrophage is the major cell to synthesis PGE2 and TXB2. The production of PGE2 and TXB2 can be regulated by different agents. In this study, elicited rat peritoneal macrophage cultured with or without different agents for certain hours then the culture fluids were assayed for PGE2 and TXB2 by radioimmuno assay after extracted with acetic ester. It has been shown that rest macrophages released little PGE2 but TXBz increased more rapidly than PGE2 in a time dependent fasion for 0-4 hours. After stimulation of zymosan for 0-4 h,the level of PGE2 and TXB2 were increased in the same fasion. Carrageenan, silica, A stimulated macrophage to increase PGE2 and TXB2 production. However, indomethacin danshensu, nifedipine and baicalin all inhibited PGE2 production by macrophages induced by
出处
《中药药理与临床》
CAS
CSCD
1990年第4期31-34,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
中国科学院基金资助课题
