摘要
用α-^(32)P-dcTP标记人巨细胞病毒(Cytomegalovirus Human type,简称HCMV)DNA片段做探针,用核酸杂交方法检测CMV DNA,其敏感性达Pg水平。并抽样检测了沈阳地区分娩期妇女尿标本。在25份标本中查出HCMV DNA阳性者占6份,排毒率24％,并对实验技术及实验结果进行了讨论。
HCMV Were examined by using DNA hybridization test with cloned HCMV DNA probe. The sensitivity arrives at pg level. 25 urine specimens from woman at the time of delivery in shenyang were examined. 6 out of 25 samples showed positive result. The positive rate is 24 per cent. The DNA—DNA hybridization test is more rapid, sensitive and specific than the CMV isolation and examining CMV antibody. The method is useful tool for researching CMV.
出处
《微生物学杂志》
CAS
CSCD
1990年第Z1期55-57,共3页
Journal of Microbiology
