摘要
利用DAPI与DNA特异结合进行简单,快速而灵敏地检测细胞培养中污染的支原体,检测被支原体污染的单层细胞整个过程不到三十分钟,就能判定培养物中是否有支原体污染,本方法可以成为一般实验室的常规方法。
With the method of specific binding of DAPI and DNA, the mycoplasma contamination cell cultures are sensitively detected. In this way, the monolayer cells can be determinated if a certain cell culture has been contaminated by some mycoplasma. The process is simple and can be done within thirty minutes. This method can be widely applied as a routine determination for detecting mycoplasma contamination in laboratory cell cultures.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第S1期126-128,150,共4页
Acta Anatomica Sinica
