摘要
本研究用乙醛酸荧光组化方法和改良的嗜铬反应电镜细胞化学技术对大鼠颈上神经节的胺贮存细胞进行了研究。用乙醛酸处理后,在大部分主神经元和小强荧光(SIF)细胞观察到指示儿茶酚胺(CA)可能主要是肾上腺素(NA)存在的特异绿色荧光。SIF 细胞常以小群分布,并多数紧靠毛细血管。主神经元的荧光从弱到中等强度,而 SIF 细胞荧光极强,并观察到肾上腺素能细胞突起的荧光膨体网。用利血平处理动物之后,主神经元的 NA 特异荧光显著减弱,表明非颗粒的 NA 更易为利血平所消耗。由特异的嗜铬反应电镜细胞化学技术揭示了主神经元含有散在的小致密芯小泡。根据我们的荧光组化和超微结构观察,存在两种类型的 SIF 细胞,它们在形态学上与 Williams 提出的Ⅰ型 SIF 细胞相当于中间神经元和Ⅱ型 SIF 细胞是内分泌机能的细胞相一致。对它们的机能意义和所含的化学物质进行了讨论。
This study employed the glyoxylic acid fluorescence histochemical method and the modified chromaffin reaction electron microscope cytochemical tech- nique to study amine storage cells of superior cervical ganglia of the rat. After glyoxylic acid treatment,a green fluorescent colour indicating the pr- esence of catecholamines(CA)was observed in the majority of the principal ganglionic neurons(PGN)and the small intensely fluorescent cells(SlF cells),often lying in small groups,as seen in single sections mostly close to capillaries.Whereas the fluorescence intensity in PGN was from weak to medium,the SIF cells were strongly fluorescent.A network of fluorescent varicosities of adrenergic cell processes were also observed.After the treat- ment of animals by reserpine the specific green fluorescence indicating the presence of CA most likely noradrenatin(NA)in PGN was greatly decreased. Obviously,the nongranular bound NA appear to be more sensitive to the depleting action of reserpine. By the specific chromaffin reaction electron microscopic cytochemical technique the PGN revealed to contain the small dense cord vesicles. According to our fluorescence histochemical and ultrastructural observa- tion,there exist two types of SIF cells.In morphology they correspond with Williams raised type Ⅰ SIF cells could be equivalent to interneurons and type Ⅱ SIF cells could be endocrine function cells.Their functional signific- ance and the chemical substances contained is discussed.
出处
《广州医学院学报》
1990年第4期6-14,共9页
Academic Journal of Guangzhou Medical College
关键词
颈上神经节
胺贮存细胞
荧光组织化学方法
电镜细胞化学技术
主神经元
小强荧光细胞
superior cervical genglia
amine storage cells
fluorescence histochemical method
electron microscope cytochemical technique
principal ganglionic neuron
small intensely fluorescent cells
