摘要
目的:探讨棕榈酸(PA)形成的高脂环境对大鼠成肌细胞糖代谢的影响,为深入研究2型糖尿病的发生机制提供理论依据。方法:用0.1、0.3、0.5 mmol/L棕榈酸处理原代培养大鼠成肌细胞6、12、24 h后,用MTT法检测成肌细胞活力;用油红O染色法检测成肌细胞脂肪变性后甘油三酯沉积;用甘油磷酸氧化酶-过氧化物酶(GPO-POD)法测定成肌细胞脂肪变性后甘油三酯含量;用同位素示踪法检测葡萄糖摄取。结果:随着PA处理浓度(0.1-0.5 mmol/L)的增加,及暴露时间(6～24 h)的增加,成肌细胞活力逐渐降低、甘油三酯沉积及含量不断增高、葡萄糖摄取不断降低,并呈现剂量和时间依赖性的效应关系。与对照组比较,0.3 mmol/L PA(24 h)和0.5 mmol/L PA(12、24 h)造成细胞活力、甘油三酯沉积及含量、葡萄糖摄取的改变最为明显。结论:PA可以作用于大鼠成肌细胞,使其脂肪沉积,甘油三酯含量增加,糖摄取减少,并呈剂量依赖关系。
AIM： To explore the effects of high-fat environment on glucose metabolism in rat myoblasts.METHODS： The rat myoblasts were exposed to palmitic acid（PA） at concentrations of 0.1 mmol/L,0.3 mmol/L or 0.5 mmol/L for 6 h,12 h or 24 h.The viability of the cells was determined by MTT assay.The oil red O dyeing method was used to display triglyceride（TG） sediment in the cells.Triglyceride content in the cells was measured by glycerophosphate oxidase-peroxidase（GPO-POD） method.The uptake of 2-deoxy-D- glucose（-G） in rat myoblasts was determined by isotope tracer method.RESULTS： After exposed to PA at concentrations of 0.1~0.5 mmol/L for 6~24 h,the viability of rat myoblasts decreased,TG sediment and content increased,and-G uptake was inhibited in a concentration-and time-dependent manner.Compared with control group,the cell viability,TG sediment and content,and -G uptake were significantly changed in 0.3 mmol/L PA group（24 h） and 0.5 mmol/L PA groups（12 h and 24 h）.CONCLUSION： With the elevating concentration of PA exposure,TG sediment increases and glucose uptake decreases in rat myoblasts.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第11期2156-2159,共4页
Chinese Journal of Pathophysiology
基金
辽宁省教育厅科研项目计划资助项目(No.L2010598)
关键词
棕榈酸
成肌细胞
甘油三酯类
糖代谢
Palmitic acid
Myoblasts
Triglycerides
Glucose metabolism
