摘要
目的:探讨Th17/Treg细胞在不明原因复发性流产患者外周血、子宫蜕膜组织中的变化以及Th17细胞相关细胞因子IL-17、IL-6的表达情况。方法:应用流式细胞术检测20例不明原因复发性流产患者(实验组)、20例正常早期妊娠人工流产患者(对照组)外周血、子宫蜕膜组织中Th17细胞和Treg细胞占CD4+T淋巴细胞的比例。ELISA法检测两组患者血清中IL-17和IL-6的浓度,Q-PCR检测两组患者子宫蜕膜组织中IL-17和IL-6mR-NA的表达。结果:20例实验组患者外周血、子宫蜕膜组织中Th17细胞占CD4+T淋巴细胞的比例为(1.9±0.3)%,(1.5±0.4)%,高于20例对照组患者的(1.0±0.2)%、(0.5±0.1)%,实验组患者中Treg细胞占CD4+T细胞的比例分别为(5.2±1.1)%、(7.5±1.4)%,低于对照组患者的(8.8±1.3)%、(12.1±1.3)%(P<0.01)。20例实验组患者血清及子宫蜕膜组织中IL-17和IL-6的表达水平高于对照组。结论:不明原因复发性流产患者中Treg细胞降低,Th17细胞增加并高分泌IL-17和IL-6,这可能是流产的重要原因。
Objective： To investigate the changes of the proportion and cytokine production of Th17 cells and the frequency of Treg cells in the peripheral blood and decidua in unexplained recurrent spontaneous abortion（URSA） patients.Methods： Flow cytometry was used to assess the percentages of Th17 cells and Treg cells in CD4＋ T cells in the peripheral blood and decidua of 20 patients with URSA and 20 health subjects undergone artificial abortion（control subjects）.ELISA and quantitative PCR were used to analyze the expression of IL-17 and IL-6 in the serum and decidua in URSA patients and control subjects.Results： URSA patients had higher percentages of Th17 cells in CD4＋ T cells in the peripheral blood and decidua（[1.9±0.3] % and [1.5±0.4] % respectively,vs.[1.0±0.2] % and [0.5±0.1] % respectively in control group,both P0.01）.However,the percentages（all P 0.01） of Treg cells in CD4＋ T cells in the peripheral blood and decidua（[5.2±1.1] % and [7.5±1.4] %） in URSA groups were lower than in control subjects（[8.8±1.3] % and [2.1±1.3] % respectively）.The expression of IL-17 and IL-6 in the serum and decidua in URSA patients was significantly higher than in control group.Conclusion： Th17 cells might play important roles in URSA through higher IL-17 and IL-6 expression.Meanwhile Treg cells may benefit the maintenance of pregnancy.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2011年第6期773-777,共5页
Medical Journal of Wuhan University
基金
湖北省自然科学基金资助项目(编号:2010CDB06204)
