摘要
目的建立HPLC同时测定连翘中连翘苷、芦丁和槲皮素的含量。方法采用ZORBAX SB C18(4.6 nm×250 mm,5μm)色谱柱;以甲醇-0.2%磷酸水溶液为流动相,梯度洗脱(0～40 min,40%～50%甲醇);连翘苷检测波长为280 nm,芦丁和槲皮素检测波长为370 nm;流速为1.0 mL.min-1;柱温为25℃。结果 3个成分均达到良好分离,连翘苷、芦丁和槲皮素平均加样回收率分别为98.2%,99.7%,100.9%。结论本检测方法简便,准确性和重现性良好,可以作为同时测定连翘中连翘苷、芦丁和槲皮素含量的方法。
OBJECTIVE To develop a HPLC method for the determination of forsythin, rutin and quercetin in Forsythia suspense （Thunb.） Vahl. METHODS These three constituents were determined by HPLC on ZORBAX SB Cl8 （4. 6 mm x 250 mm, 5 I.Lm） column with the mixture of CH3 OH-0. 2% H3PO4 as the mobile phase. Forsythin was detected at 280 nm, and rutin and quercetin were detected at 370 nm. The flow rate was 1.0 mL·min^-1 and the column temperature was 25℃. RESULTS The recoveries were 98.2% with RSD of 2. 8% for forsythin, 99.7% with RSD of 3.2% for rutin, and 100. 9% with RSD of 2. 7% for quercetin. CON- CLUSION The method is simple, accurate and reproducible. The established method can be used for the determination of forsythin, rutin and quercetin in Forsythia suspense（Thunb. ）Vahl.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2011年第22期1756-1758,共3页
Chinese Pharmaceutical Journal
基金
教育部科学技术研究重点资助项目(DF2003078)
关键词
连翘
连翘苷
芦丁
槲皮素
高效液相色谱法
Forsythia suspense （ Thunb. ） Vahl
forsythin
rutin
quercetin
HPLC
