摘要
目的:通过滇重楼总皂苷含量和AM菌根侵染率与根际土壤性质的相关研究和通径分析,探讨土壤因子在滇重楼品质形成中的作用。方法:测定了25个主要分布区滇重楼根茎总皂苷含量、土壤理化性质和养分状况、根系AM菌根侵染率,并应用相关分析和通径分析研究了滇重楼总皂苷含量和菌根侵染率与土壤因子的关系。结果:在自然条件下,滇重楼根系能与AM真菌形成良好的共生关系,侵染率在36.41%～83.37%。总皂苷与脲酶活性、碱性磷酸酶活性、有机质含量呈显著正相关,与土壤容重呈显著负相关。AM菌根侵染率与碱解氮呈显著正相关。通径分析结果表明,滇重楼总皂苷含量主要受其根际土壤碱解氮的影响,其次为土壤有机质和脲酶活性。而菌根侵染率主要受土壤pH制约,其次为碱解氮、脲酶活性和速效磷。结论:滇重楼品质形成与土壤因子密切相关,通径分析更能反映土壤因子对滇重楼总皂苷含量和菌根侵染率的贡献。
Objective： Through correlation and path analysis between total saponins content in rhizome/mycorrhizal infection rate in roots of Pairs polyphylla var.yunnanensis and soil factors,to make an inquiry into the role of soil factors in the quality formation of P.polyphylla var.yunnanensis.Method： Tested total saponins in rhizome,mycorrhizal fungal infection rate in root and physical and chemical properties in rhizosphere soil in 25 different growth areas,and statistically analyzed the relationship between total saponins in rhizome/mycorrhizal infection rate in roots of P.polyphylla var.yunnanensis and soil factors by using correlation and path analysis.Result： The symbiosis relationship between AM mycorrhizal and roots of P.polyphylla var.yunnanensis were better established under natural condition,of which the infection ratio between 36.41%,83.37%.There were significantly positive correlation between total saponins content in rhizome and urease activity or alkaline phosphatase activities or organic matter in soil,but there was significantly negative correlation between total saponins content and bulk density.There was significantly positive correlation between AM infection ratio and alkaline nitrogen.Path analysis indicated that total saponins of rhizome mainly affected by alkaline nitrogen in soil rhizosphere,secondly by soil organic matter and soil urease activity.While the mycorrhizal fungal colonization ratio was mainly affceted by soil pH,secondly by alkaline nitrogen,urease activity,and available phospherus in soil.Conclusion： There is closed relationship between quality formation of P.polyphylla var.yunnanensis and soil factors.Path analysis is better for reflecting the contribution of soil factors to total saponins and mycorrhizal infection ratio.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第22期3091-3095,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30873374)
关键词
滇重楼
皂苷
AM菌根
土壤因子
通径分析
Pairs polyphylla var.yunnanensis
total saponins content
AM mycorrhizal
soil factors
path analysis
