摘要
目的探讨鼠神经干细胞对鼠胶质瘤C6细胞侵袭能力的影响及机制。方法取新生1～2天SD大鼠大脑皮层组织,于无血清培养基中分离培养神经干细胞并进行NES免疫细胞化学染色,并将神经干细胞和鼠胶质瘤C6细胞于体外共培养,利用2D、3D生长实验和Transwell实验分别检测与鼠神经干细胞共培养鼠胶质瘤C6细胞(实验组)和单独培养鼠胶质瘤C6细胞(对照组)的侵袭能力。结果 2D实验中C6细胞的对照组和实验组的平均直径分别为(18.53±1.32)μm和(13.44±1.45)μm,差异有统计学意义(P<0.01)。3D实验中C6细胞的对照组和实验组的平均直径分别为(20.34±1.25)μm和(15.52±1.43)μm,差异有统计学意义(P<0.01)。Transwell侵袭实验发现胶质瘤C6细胞组中对照组和实验组平均视野侵袭细胞为(36.45±1.36)个和(22.73±1.66)个,差异有统计学意义(P<0.01)。结论与神经干细胞共同培养后C6细胞的侵袭能力明显降低。
Purpose To explore the impact and mechanism of neural stem cells of rat on the invasiveness of C6 glioma cells of rat.Methods The cerebral cortices of newborn 1-2 day Sprague-Dawley rats（SD rats） were extracted,then the neural stem cells in serumfree medium were separated and cultured.These cells were stained with NSE immunohistochemical staining.After that the neural stem cells were co-cultured with C6 glioma cell of rat in vitro.The invasiveness of neural stem cells of rat which is co-cultured with C6 glioma cell of rat（the experimental group） and individually cultured C6 glioma cell of rat（the control group） were detected by utilizing 2D 3D growth experiments and Transwell experiments respectively.Result In two-dimensional growth experiment,the average diameter of C6 cells in control group was（18.53±1.32） μm,however,in experimental group it was（13.44±1.45） μm.The difference has statistical significance（P0.01）.In three-dimensional growth experiment,the average diameter of C6 cells in control group was（20.34±1.5） μm,however,in experimental group it was（15.52±1.43） μm.The difference has statistical significance（P0.01）.In the Transwell invasion experiment,the average number of membrane-spanning cells was（36.45±1.36） in control group compared with（22.73±1.66） in experimental group.The difference has statistical significance（P0.01）.Conclusion After co-culturing with neural stem cells,the invasiveness of C6 cells decreases obviously.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2011年第11期1185-1188,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
黑龙江省教育厅科学技术研究项目(12511174)
