摘要
2009年8月,山西榆次某鸡场蛋种鸡群产蛋量急剧下降,部分鸡产蛋停止。病理组织学观察发现,病鸡肝脏、肾脏、肺脏、心肌等组织中出现小灶状淋巴细胞瘤,肿瘤细胞为成熟的淋巴细胞;聚合酶链式反应(PCR)、酶联免疫吸附试验(ELISA)和间接免疫荧光(IFA)检测显示,被检鸡ALV-J感染率为8/8,而且7/8为病毒血症,与REV共感染率为2/8,未出现REV单感染;从发病情况看,淋巴细胞瘤均出现于共感染病例,特别是ALV-J病毒血症的共感染鸡病变最为严重,但未见ALV-J的特征性髓细胞瘤。囊膜蛋白氨基酸同源性分析显示,ALV-J毒株与英国HPRS-103原型株同源性最高(96.9%以上),共感染的REV毒株与suv-env株同源性最高(93.8%)。本病例揭示,先天传播的ALV-J引起的病毒血症状态可能为REV的致瘤创造了环境,使临床罕见的REV淋巴瘤早于髓细胞瘤出现,因此,严格种鸡群的ALV-J净化是防控此病的重中之重。对于ALV-J与REV协同的机制还需进一步研究。
An outbreak of productive diseases occurred in a commercial layer breeding company in Yuci,Shanxi Province during August 2009.Lymphocytoma presented in lung,liver,kidney,heart,etc.ELISA,PCR and IFA results indicated that the positive rate of serum antigen to ALV-J was 8/8 and the viremia rate was 7/8.The co-infection rate with REV was 2/8,and found no single REV infection.The pathogenic observation indicated that the most serious lymphocytoma presented in co-infected viremia chicken,but no myelocytomas were observed.Phylogenitic analysis showed that the homology was over 96.9% of three strains of ALV-J with the prototype HPRS-103;The REV strain showed the highest homology with suv-env strain（93.8%）.The case revealed that viremia state induced by ALV-J is necessary for lymphocytomas formation induced by REV,and it is the reason why lymphocytomas showed earlier than myelocytomas.So,it is critical to eliminate vertical transmission of ALV-J for prevention these diseases.The synergistic effect mechanism of ALV-J and REV need to be further study.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2011年第11期1643-1648,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山东省自然科学基金(Y2007D46)
山东省科技攻关(2010GNC10943)
