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SKI-Ⅱ对胃癌SGC7901细胞血管内皮生长因子表达的影响 被引量：1

Effects of SKI-Ⅱon the expression of vascular endothelial growth factor in SGC7901 gastric cancer cells in vitro
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摘要 目的观察SKI-Ⅱ对胃癌SGC7901细胞内血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,并对其机制进行初步研究。方法常规培养胃癌SGC7901细胞,给予不同浓度SKI-Ⅱ及顺铂(DDP)干预,MTT法测定各组胃癌SGC7901细胞增殖情况,免疫组化和Western blot检测药物对SphK1、VEGF表达的影响。结果 MTT检测显示SGC7901细胞与不同浓度的SKI-Ⅱ共同培养,细胞的生长受到不同程度的抑制,其作用具有剂量依赖性,并随时间的延长逐渐增强,免疫组化及Western blot检测显示SKI-Ⅱ可显著下调肿瘤细胞SphK1和VEGF蛋白的表达,单用DDP对SphK1、VEGF无作用。结论 SKI-Ⅱ可以通过下调VEGF和SphK1蛋白表达而有效抑制胃癌SGC7901细胞增殖。 Objective To investigate the effects and mechanism of SKⅠ-Ⅱ on the expression of vascular endo- thelial growth factor （VEGF） in gastric cancer SGC -7901 cells. Methods SGC7901 gastric cancer cells in vitro were treated with different concentrations of SKⅠ-Ⅱ or DDP. Cell proliferation was analyzed by MTT assay. And SphK1 and VEGF protein expression was assessed by immunohistochemistry and western blot. Results SGC7901 cell proliferation was inhibited by SKⅠ-Ⅱ in dose - dependent and time - dependent manners. The expression of SphK1 and VEGF in SGC7901 cells was reduced by SKⅠ-Ⅱ , but not by DDP. Conclusion SKⅠ-Ⅱ down - regulates SphK1 and VEGF proteins, thus inhibits the proliferation of SGC7901 cells.
作者 周红连 费素娟 朱祖安 孙旻 张秋月
机构地区 徐州医学院附属医院消化内科
出处 《广东医学》 CAS CSCD 北大核心 2011年第17期2229-2231,共3页 Guangdong Medical Journal
关键词 SKI-Ⅱ 血管内皮生长因子 SphK1 SGC7901细胞 SKⅠ-Ⅱ vascular endothelial growth factor SphK1 SGC7901 cells
分类号 R735.2 [医药卫生—肿瘤]
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参考文献15

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广东医学
2011年 第17期

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