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抗ICAM-1单克隆抗体的制备与特性鉴定 被引量：2

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摘要 目的:制备有生物学活性的抗人细胞间黏附分子-1(ICAM-1)单克隆抗体(mAb)并进行特性鉴定。方法:以纯品ICAM-1为抗原免疫BALB/c小鼠4次。采用杂交瘤技术,经3次亚克隆筛选稳定分泌抗人ICAM-1 mAb的杂交瘤细胞株。采用动物体内诱生的方法大量制备mAb。以protein G对其进行纯化后,用间接ELISA法测定mAb的效价并鉴定其Ig亚类。用Western blot鉴定mAb的特异性。结果:筛选出4株可稳定分泌抗人ICAM-1 mAb的杂交瘤细胞株,分别命名为B9株、F1株、C11株和D6株。4株mAb的Ig亚类均为IgG1。4株mAb培养上清的效价均为1∶1 000;腹水的效价B9株与D6株为1∶2×105,F1株与C11株为1∶4×105。纯化后mAb的蛋白浓度为1.2 g/L,均可与ICAM-1特异性结合。结论:成功制备出效价高、特异性良好的4株抗ICAM-1 mAb,为进一步研究ICAM-1的生物学功能和临床应用奠定了基础。
作者 柴锦燕 方佩华 李宁 董升
机构地区 天津医科大学总医院核医学科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2011年第11期1212-1214,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 天津市科技发展计划项目(05YFGDSF02700)
关键词 细胞间黏附分子 1 单克隆抗体 杂交瘤技术
分类号 R392.12 [医药卫生—免疫学]
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参考文献12

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二级参考文献9

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细胞与分子免疫学杂志
2011年 第11期

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