摘要
目的建立减肥保健食品中非法添加化学成分酚酞的检测方法。方法采用LC-MS/MS联用技术,采用Zorbax Eclipse Plus C18色谱柱,以含0.1%冰醋酸的20mmol/L醋酸铵溶液为流动相A,以甲醇为流动相B,梯度洗脱,流速:0.2mL/min;柱温为35℃,电喷雾电离,正离子模式,多重反应监测扫描方式对酚酞的母离子(m/z319)及其子离子(m/z197和225)进行检测。结果建立检测减肥保健食品中非法添加的酚酞的检测方法,检测限低于0.06ng。在抽取的70批次市售减肥保健食品中,19批次检出了酚酞。结论该方法专属性强、灵敏度高,可用于减肥保健食品中非法添加的酚酞的筛选与确证工作。
Objective To develop a LC-MS/MS method for the identification of illegal addition of chemical substance Phenolphthalein in health food.Methods LC-MS/MS method was adopted.The separation was achieved by using Zorbax Eclipse Plus C18 column with gradient eluent composed of 2 mmol/L ammonium acetate containing 0.1% acetic solution-methanol.The flow rate was 0.2 mL/min and the column temperature was set at 35℃.The target compound was analyzed with mass spectrum in the Multiple-reaction monitoring mode via positive ion mode.Results The illegal addition of chemical substance Phenolphthalein in health food was detected on the basis of mass spectra.The LOD was below 0.06 ng.Among the 70 samples,19 samples contained Phenolphthalein.Conclusion The method is sufficiently selective and sensitive to detect the Phenolphth alein in health food.
出处
《中国医药科学》
2011年第18期98-100,共3页
China Medicine And Pharmacy
