摘要
目的:探讨超敏C-反应蛋白(hsCRP)、肿瘤坏死-α(TNF-α)、白细胞介素-10(IL-10)对小儿败血症的早期诊断价值。方法:选择临床诊断为败血症患儿57例和同期无感染征象的小儿36例,采用双抗体夹心ELISA法检测血清TNF-α,IL-10的水平,采用速率散射比浊法测定hsCRP。结果:hsCRP、IL-10和TNF-α在败血症组和对照组有显著意义。hSCRP有较高的特异度;TNF-α的敏感度是最高的。联合诊断时hsCRP和TNF-α的临床诊断价值最高。结论:hsCRP、IL-10和TNF-α对小儿败血症的早期诊断有一定价值;hsCRP和TNF-α是最佳的联合诊断指标之一。
Objective：To investigate the value of high sensitivity C-reactive protein（hsCRP）,tumor necrosis factor-α（TNF-α）,interleukin（IL-10）in early diagnosis of pediatric sepsis.Methods： 57 children with pediatric sepsis and 36 children without signs of infection were chosen for measuring serum hsCRP,TNF-α and IL-10.The TNF-α and IL-10 were determined by double-antibody sandwich ELISA technique and the hsCRP was determined by rate nephelometry assay.Results： The level of hsCRP,TNF-α,IL-10 in pediatric sepsis and the controls had significant difference.hsCRP had high specificity while TNF-α had the highest sensitivity.hsCRP and TNF-α co-diagnosis in pediatric sepsis were found better.Conclusion： Determination of hsCRP,TNF-α and IL-10 is valuable for the diagnosis of pediatric sepsis;hsCRP and TNF-α were combined to improve the diagnostic sensitivity,which is one of the best indicators of the joint diagnosis.
出处
《中国卫生检验杂志》
CAS
2011年第11期2710-2711,共2页
Chinese Journal of Health Laboratory Technology
