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重组人甲状旁腺素（1-34）对肿瘤坏死因子-α诱导的HaCaT细胞增殖及细胞周期的影响 被引量：3

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摘要 目的观察重组人甲状旁腺素（1-34）[rhPTH（1-34）]对肿瘤坏死因子-ct（TNV-α诱导的永生化人角质形成细胞（HaCaT）增殖的影响。方法不同浓度rhPTH（1-34）作用于由TNF-α诱导的HaCaT细胞，观察细胞形态变化；噻唑蓝法检测不同浓度rhFFH（1-34）作用后细胞增殖情况；流式细胞检测药物作用后细胞周期变化。结果相差显微镜下可见，HaCaT细胞经rhPTH（1-34）作用后，细胞生长状态和生长速度受抑制；噻唑蓝法显示rh胛H（1-34）（0．05、0．2、0．8、3．2、12．8Ixmol／L）分别作用HaCaT细胞36h、48h后，细胞增殖受到明显抑制（P〈0．05或0．01）；流式细胞检测显示，rhPTH（1-34）（0．2、0．8、3．2、12．8tzmol／L）作用细胞48h后，G1期细胞比例明显增加（P值均〈0．01），s期比例下降（P值均〈0．01）。结论在体外rhPTH（1-34）对TNF-0诱导的HaCaT增殖有明显抑制作用，且呈剂量依赖性。 Objective To investigate the influence of recombinant human parathyroid hormone [rhPTH (1-34)]on the proliferation of HaCaT cells induced by tumor necrosis factor-α (TNF-α) in vitro.Methods Cultured HaCaT cells were treated with various concentrations of rhPTH (1-34) for different durations after incubation with recombinant human TNF-α of 10 g/L for 24 hours.MTT assay and flow cytometry were performed to detect the proliferation and cell cycle of HaCaT cells,respectively.Results As contrast phase microscopy showed,the growth of HaCaT cells was inhibited by rhPTH (1-34) along with a decrease in the growth speed.MTT assay showed a suppressed proliferation of HaCaT cells after being treated with rhPTH (1-34) of 0.05,0.2,0.8,3.2 and 12.8 pmol/L for 36 and 48 hours (P＜ 0.01 or 0.05).The percentage of cells at G1 phase in HaCaT cells markedly increased (all P ＜ 0.01 ),while that at S phase declined (all P ＜ 0.01 )after 48-hour treatment with rhPTH(1-34) of 0.2,0.8,3.2 and 12.8 μ mol/L.Conclusions rhPTH(1-34) has an obvious inhibitive effect on the proliferation of HaCaT cells induced by TNF-α in vitro,and the effect is in a dose-dependent manner.
作者 卜晓琳 毕新岭 史玉玲 汪五清 顾军
机构地区 上海第二军医大学长海医院皮肤科
出处 《中华皮肤科杂志》 CAS CSCD 北大核心 2011年第12期871-873,共3页 Chinese Journal of Dermatology
基金 上海市科委基础研究重点项目（08JCl406800）,杨森科学基金（2008）
关键词 角蛋白细胞 甲状旁腺素 银屑病 细胞增殖 细胞周期
分类号 R285 [医药卫生—中药学]
  • 相关文献

参考文献9

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二级参考文献16

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共引文献6

同被引文献32

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二级引证文献3

中华皮肤科杂志
2011年 第12期

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