摘要
目的:探讨D-半乳糖联合染铝的阿尔茨海默病大鼠海马结构Aβ及其相关蛋白的表达及其分布。方法:30只雄性SD大鼠随机分为2组,即:对照组和模型组(n=15)。模型组每日腹腔注射D-半乳糖60 mg/kg和灌胃三氯化铝500 mg/kg,连用60 d;对照组给予等量生理盐水进行腹腔注射和灌胃。铃木镀银法观察神经原纤维缠结的形成,确定模型是否成功。免疫组织化学法观察海马结构三个亚区的Aβ、β分泌酶(BACE)和早老素-1(PS-1)蛋白的表达。结果:铃木镀银染色可见模型组大鼠颞叶、顶叶皮层及海马神经元内的神经原纤维缠结。模型组大鼠海马CA1、CA3区及齿状回Aβ表达较强,与对照组相比差异显著(P<0.01);模型组海马CA3区神经元的BACE和PS-1蛋白表达量较对照组明显增加(P<0.01)。结论:D-半乳糖联合染铝的阿尔茨海默病大鼠海马结构Aβ、BACE及PS-1表达均明显上调,可能是导致AD大鼠功能障碍的主要原因。
Objective： To observe the expression and distribution of β-amyloid protein（Aβ） and related protein in hippocampus of Alzheimer＇s disease（AD）-like rats treated with D-galactose and aluminium trichloride.Methods： Thirty male Sprague-Dawley rats were randomly divided into control and model groups with 15 each.Model rats were treated with D-galactose（60 mg/kg/d,ip） and aluminium trichloride（500 mg/kg/d,ig） for 60 days.Rats in control group were treated with the same dose of physiological saline as control group.Silver staining for neurofibrillary tangles was used to assess the success of AD model.Immunohistochemical staining was performed to detect the expression of Aβ,β-site APP cleaving enzyme（BACE） and presenilin-1（PS-1） in hippocampal formation.Results： Silver staining showed the neurofibrillary tangles in neurons of temporal and parietal lobes and hippocampus in the rats of model group.The expression of Aβ was sighificantly increased in CA1,CA3 and dentate gyrus in model rats（P〈0.01,compared to control）.The expression of BACE and PS-1 in hippocampal CA3 region of model rats was significantly increased compared with that in control group（P〈0.01）.Conclusion： The expression of Aβ,BACE and PS-1 in hippocampal formation of Alzheimer＇s disease-like rats treated with D-galactose and aluminium trichloride shows up-regulation,which may be the main reason leading to AD rats dysfunction.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2011年第6期633-636,共4页
Chinese Journal of Neuroanatomy
基金
辽宁省科学技术计划项目(2010225034)
