摘要
用循环伏安法制备铜掺杂聚L-酪氨酸修饰玻碳电极,研究了尿酸、抗坏血酸和多巴胺在修饰电极上的电化学行为,建立了同时测定尿酸、抗坏血酸和多巴胺的新方法。在pH 3.0磷酸盐缓冲溶液中,扫描速率为140 mV/s,多巴胺在修饰电极上产生一对氧化还原峰,峰电位分别为0.502 V、0.370 V,尿酸和抗坏血酸分别产生一氧化峰,峰电位分别为0.653 V和0.253V。尿酸、多巴胺的氧化峰分开达0.151 V,多巴胺和抗坏血酸的氧化峰分开达0.249 V,采用循环伏安法(CV法)和示差脉冲伏安法(DPVs法),用氧化峰可同时测定尿酸、多巴胺和抗坏血酸。CV法和DPVs法同时测定的线性范围分别为:UA:3.00×10-6~1.00×10-4mol/L(CV)、3.00×10-7~1.00×10-4mol/L(DPVs);DA:1.00×10-6~1.00×10-4mol/L(CV)、1.00×10-7~1.00×10-5mol/L、1.00×10-5~1.00×10-4mol/L(DPVs);AA:3.00×10-5~1.00×10-3mol/L(CV)、3.00×10-6~1.00×10-3mol/L(DPVs)。检出限分别为3.0×10-7、1.0×10-7、3.0×10-6 mol/L(CV)和1.0×10-8、1.0×10-8、1.0×10-7 mol/L(DPVs)。方法可用于人尿液和血清中尿酸、多巴胺和抗坏血酸的同时测定。
A copper doped poly(L-tyrosine) modified electrode was prepared by cyclic voltammetric method.The voltammetric behavior of uric acid(UA),dopamine(DA) and ascorbic acid(AA) and cyclic voltammetric method for the simultaneous determination of UA,DA and AA were studied at the modified electrode.In pH 3.0 phosphate buffer solution,the modified electrode gave a pair of redox peaks at Epa=0.502V,Epc=0.370V for DA,one oxidation peak at Epa=0.653V for UA and one oxidation peak at Epa=0.253V for AA,respectively.Two separated oxidation peaks were divided 0.151V for UA and DA,0.249V for AA and DA.The linear responses were obtained in the range of 3.00×10-6~1.00×10-4 mol/L(CV) and 3.00×10-7~1.00×10-4 mol/L(DPV) for UA,1.00×10-6~1.00×10-4 mol/L(CV) and 1.00×10-7~1.00×10-5 mol/L,1.00×10-5~1.00×10-4 mol/L(DPV) for DA and 3.0×10-5~1.0×10-3mol/L(CV) and 3.00×10-6~1.00×10-3 mol/L(DPV) for AA.The detection limits of UA,DA and AA were 3.0×10-7 mol/L,1.0×10-7 mol/L,3.0×10-6 mol/L(CV) and 1.0×10-8 mol/L,1.0×10-8 mol/L,1.0×10-7 mol/L(DPVs).The method was successfully applied to the simultaneous determination of UA、 DA and AA in human urine and serum samples with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2011年第12期45-50,共6页
Chinese Journal of Analysis Laboratory
基金
安徽省高校省级自然科学研究重点项目(KJ2008A122
KJ2011A255)资助
关键词
L-酪氨酸
尿酸
多巴胺
抗坏血酸
铜掺杂
化学修饰电极
同时测定
L-Tyrosine
Uric acid
Dopamine
Ascorbic acid
Copper doped
Chemically modified electrode
Simultaneous determination