摘要
选用20对SSR引物对2007-2008年间从云南省56个县(区)收集的416份地方稻种进行遗传多样性分析。共检测到116个等位变异,每个位点变幅为3～13,平均为5.8个;SSR引物多态性信息含量PIC指数平均为0.681,最小为RM18的0.349,最大的RM6748为0.891;56个县的PIC变幅为0.025～0.679,平均为0.381,勐海县的PIC最高,广南县等仅有1份参试材料的8个县的PIC最低。聚类结果表明,在遗传一致度为0.362处将56个县的稻种分为3个类群,第Ⅰ类包括勐海等54个县,在阈值为0.499处又分为2个亚类,其中亚类Ⅰ-1包括36个县,亚类Ⅰ-2包括18个县,第Ⅱ类仅包括思茅区,第Ⅲ类仅包括河口县;楚雄州的牟定县与姚安县的遗传一致度最大,为0.943,大姚县与姚安县的遗传一致度也较高,为0.925,思茅区与宁蒗县的遗传一致度最小,为0.033,各县(区)的遗传一致度与地理位置有一定的相关性。本研究结果表明,云南当前种植的地方稻种具有丰富的遗传多样性,但分布很不均匀,遗传多样性主要集中在西双版纳、德宏、普洱、临沧和红河5个州市。
A total of 416 varieties being grown during 2007-2008 in 56 counties in Yunnan province were evaluated using 20 pairs of SSR markers.Total of 116 alleles were detected and the number of alleles per marker ranged from 3 to 13,with an average of 5.8.The mean of polymorphism information content（PIC）of SSR markers was 0.681,with a range from 0.349 at RM18 to 0.891 at RM6748.PIC varied from 0.025 to 0.679 among the 56 counties,with an average of 0.381,that of Menghai was the highest while that of eight counties in each only one accession being collected was the lowest.Cluster analysis with UPGMA method based on genetic identity（GI）indicated that 56 counties were classified into 3 groups at GI of 0.362,GI was 0.943,the highest between Mouding and Yao＇an counties while that was the lowest between Simao and Ninglang counties,with GI of 0.033.GI was related to geographical regions among 56 counties.Rich genetic diversity exists in Yunnan rice landraces in present production.Genetic diversity of landrace rice distribute uniformly between prefectures in Yunnan province and rich genetic diversity mainly concentrate in five prefectures including Xishuangbanna,Dehong,Puer,Lincang and Honghe.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2011年第6期890-895,共6页
Journal of Plant Genetic Resources
基金
科技基础性工作专项(2006FY110702)
云南省科技创新强省计划项目(2007C0219Z)
云南省重点基金项目(2010CC009)
国家"863"项目"绿色超级稻新品种培育"
云南省人才培养项目(2008PY049)
