摘要
利用与穗行数有关的5个导入系及轮回亲本综3进行GriffingⅣ双列杂交发展分离群体,结合SSR标记和田间表型鉴定,分析玉米穗行数QTL及其相互作用。在导入系×综3所发展的5个F2群体中,仅在一个群体中检测到1个穗行数QTL,所解释的表型变异为10.68%。在导入系间杂交所发展的F2群体中检测到9个QTLs,分别位于第13、、8染色体上,所解释的表型变异在4.53%～6.52%之间。另外,检测到2对QTL间互作,10对QTL与未检测到QTL的导入片段间的互作,单个F2群体中各类互作所解释的表型变异显著大于QTL所解释的表型变异。这些结果表明,基因互作在玉米穗行数形成中起着重要的作用。
Five introgression lines（ILs）and Zong3 were selected to develop segregated populations by Griffing Ⅳ diallel crossing for identifying of QTL for row number per ear and assay of QTL interaction.The SSR markers were used to screen genotype of ILs and F2 individual.In the five IL ×Zong3 F2 populations,only one QTL for row number per ear was detected,with 10.68% phenotypic variation.In populations derived from crossing among ILs,total of 9 QTLs were detected,on chromosome 1,3 and 8,with 4.53% to 6.52% phenotypic variation.In addition,2 pairs interactions between QTLs,10 pairs interactions between QTLs and introgressed fragments without QTLs were detected.The sum of phenotypic variation explained by all of interaction pairs was significantly higher than that explained by QTLs in each of F2 populations.These results indicated that epitasis plays an important role in genetic controlling of row number per ear in maize.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2011年第6期965-970,共6页
Journal of Plant Genetic Resources
基金
国家重点基础研究计划(2009CB118400)
国家自然科学基金(30871536)
