摘要
目的:探讨缺氧诱导因子-1α(HIF-1α)基因沉默对裸鼠肺癌移植瘤放射敏感性的影响。方法:采用A549和A549/HIF-1α(-)细胞株建立人肺癌移植瘤模型,分别给予5、10、15和20Gy单次剂量X射线照射,观察放射治疗对肿瘤的抑制作用,根据肿瘤生长延缓和照射剂量分析肿瘤剂量效应及HIF-1α基因沉默对在体肿瘤放射敏感性的影响。结果:放射治疗能明显抑制肿瘤生长,并观察到随照射剂量的增加肿瘤生长延缓愈显著,接受等剂量照射HIF-1α基因沉默肿瘤生长延缓更明显,根据肿瘤剂量效应曲线计算放射增敏比分别为1.40、1.34和1.31。结论:放射治疗能显著抑制A549和A549/HIF-1α(-)肿瘤生长,A549/HIF-1α(-)肿瘤对放射治疗更敏感。
OBJECTIVE： To observe the effect of HIF-1α siliencing on human lung cancer xenografts radiosensitivity in nude mice. METHODS： The human lung cancer model in nude mice were established with A549 and A549/HIF-1α（-） cell lines and were locally irradiated with single doses of 5,10,15 and 20 Gy radiation. The tumor inhibition by radiotherapy was observed. The tumor dose-response relationship and therapeutic gain of HIF-1α siliencing were analized by tumor growth delay and radiation dose. RESULTS： Radiotherapy could inhibit tumors growth apparently. The tumor growth delay was prolonged with radiation-dose increasing. It was longer in HIF-1α siliencing group than in A549 group irradiated by the same-dose X-ray. The sensitivity enhance ment ratio were 1. 40, 1. 34 and 1. 31. CONCLUSIONS： Both A549 and A549/HIF-1α（--） tumor growth can be inhibited by ra- diotherapy. The A549/HIF-1α-） xenografts are more sensitive to radiotherapy than A549 xenografts.
出处
《中华肿瘤防治杂志》
CAS
2011年第21期1665-1668,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30570547)
山东省医药卫生科技发展计划(2009HZ078)
关键词
细胞低氧
肺肿瘤
辐射耐受性
基因沉默
小鼠
裸
cell hypoxia
lung neoplasms
radiation tolerance
gene silencing
mice, nude
