摘要
目的观察创伤弧菌脓毒症大鼠血清神经元特异性烯醇化酶（NSE）、S-100β蛋白和脑组织炎症因子的动态变化及头孢派酮钠联合左氧氟沙星的干预。方法100只sD大鼠随机分正常对照组（A组，n=10）、创伤弧菌脓毒症组（B组，n=40）、抗菌药物干预组（C组，n=40）和抗菌药物对照组（D组，n=10）左后肢皮下注射创伤弧菌构建大鼠脓毒症模型，腹腔注射头孢派酮钠180mg／kg和左氧氟沙星18ms／kg干预。观察B、c组大鼠染菌后6、12、24、48h血清NSE、s-100β，脑组织TNF-a、IL-6、IL-10转录和蛋白水平及光镜变化。结果与A组比较，B组大鼠血清NSE、S—100B蛋白均明显升高（P〈0．05），24h达峰值。与相同时间点B组比较，C组血清NSE、S—100β12、24、48h均有明显减低（P〈0．05）。与A组比较，B组大鼠脑组织各时间点TNF-a、IL-6、IL-10转录和蛋白水平均明显升高（P〈0．05），其中TNF—a在6h即达峰值，而IL-6水平12h达峰值，IL-10水平在48h达峰值。与B组相同时间点比较，6、12、24h点C组大鼠脑组织TNF-a水平明显降低，12、24、48h点脑组织IL-6水平亦明显下调，而脑组织IL-10水平在各时间点均较B组明显升高（P〈0．05）。光镜下B组大鼠脑组织间质水肿严重，组织结构紊乱，炎症细胞侵润，血管扩张。C组大鼠脑组织间质水肿减轻，炎症细胞侵润程度降低。结论NSE、S-100B蛋白的表达随着脑组织炎症的加重逐渐升高，敏感地反应创伤弧菌脓毒症脑损伤的变化。使用头孢哌酮和左氧氟沙星能有效抑制脑组织炎症反应，减轻脑损伤，减低血清NSE、S—100β蛋白的水平。
Objective To investigate Neuron- specific enolase （NSE） and S- 100β protein expressions in blood serum and TNF-a, IL- 6, IL- 10 expressions in brain tissue with VV sepsis and effect of Cefoperazone Sodium and Levofloxacin. Methods One hundred SD rats were divided into normal control group（ group A, n = 10）, sepsis - associated encephalopathy group （ group B, n = 40）, intervention of effective antibiotics （group C, n = 40） and antibiotics control group（ group D, n = 10）, build VV sepsis with injection of VV in teft lower limb, and intervention by Cefnperazone Sodium and Levofloxacin with the dose of 180 mg/kg and 18 mg/kg. Detect expressions of NSE, S - 100β protein in blood serum, TNF -a, IL -6, IL - 10 mRNA and protein in brain tissue in group B and C respectively at 6 h, 12 h, 24 h, 48 h after infection. Observe pathologic features of each group under microscope. Results Compared with group A, the level of NSE and S - 100β protein in blood serum of group B were signigicantly increased（P 〈0.05 ）, and reach the peak at 24 h. Compared with group B, the level of NSE and S - 100β protein in group C were significantly decreased at 12 h, 24 h, 48 h （P 〈 0.05）. Compared with group A,the expressions of TNF -a, IL-6, IL - 10 mRNA and protein in brain tissue in group B were significandy higher（P 〈 0.05）, and reach the peak at 6 h, 12 h, 48 h respectively. Compared with group B, the group C level of TNF - a at 6 h, 12 h, 24 h were siginificantly decreased, IL -6 at 12 h, 24 h, 48 h were siginificantly decreased, however, the level of IL- 10 were significantly increased（P 〈0.05）. Compared with group A , the brain tissue pathologic features of group B at 24 h, infiltration of inflammatory cell, edema of brain tissue in group B were significantly aggravate. Compared with group B at 24 h, tissue lesions were significantly alleviated in group C. Conclusion With the intensify of inflammation in brain tissue, the expression of NSE and S - 100β protein were increased gradually, a sensitivity to brain injury with VV sepsis. Cefoperazone Sodium and Levofloxacin can inhibit the inflammatory response, reduce brain damage,lower the level of NSE and S -100β protein.
出处
《中国急救医学》
CAS
CSCD
北大核心
2011年第12期1079-1083,I0012,共6页
Chinese Journal of Critical Care Medicine
基金
浙江省医学扶植重点建设学科计划项目（No.07-F04）
温州市科技计划项目（No.Y20090111）
