摘要
高效的化合光免疫分析与长期的耐久性，好精确并且节省时间，在人的浆液为免费 17-estradiol (E2 ) 的察觉被建议。96 microplates 与牛的浆液白朊是涂的是的结合的 E2 抗原为免疫分析的稳固的阶段。在 microplate 上涂的 E2-BSA 抗原和在样品的 E2 抗原在标记 anti-E2 抗体的山葵 peroxidase (HRP ) 上为有约束力的地点竞争了。化合光反应被催化 luminol-H2O2 底层的 HRP 随后执行，并且化合光紧张与在人的 sera 样品的 analyte 的数量相反地成正比。immunoreagents，免疫反应时间，和在免疫分析之上的另外的相关可变条件的集中被学习并且优化。建议方法展出了象 5.94 瑸慲瑣 ? 牦浯㈠‰慰楴湥獴甠楳杮栠杩 ? 敲潳畬楴湯ㄠ ? 一样低的察觉限制 ?? 灳 ' 虪光礉樀囪?潣橮湵瑣潩 ? 楷桴洠汵楴慶楲瑡 ? 湡污獹獥 ? ?档愠 ? 牰湩楣慰 ? 潣灭湯湥 ? 湡污獹獩吗？？
A high-performance chemiluminescence immunoassay, with long-term durability, good precision and time- saving, was proposed for the detection of free 17β-estradiol （E2） in human serum. Ninety-six microplates were coated with bovine serum albumin conjugated E2 antigen as solid phase for the immunoassay. The E2-BSA antigen coated on the microplate and the E2 antigen in the sample competed for the binding sites on the horseradish peroxi- dase （HRP） labeled anti-E2 antibody. Chemiluminescence reaction was subsequently carried out by HRP catalyzing luminol-H2O2 substrates, and the chemiluminescence intensity was inversely proportional to the amount of analyte in human sera samples. The concentration of immunoreagents, immunoreaction time, and other relevant variable conditions upon the immunoassay were studied and optimized. The proposed method exhibited detection limit as low as 5.94× 10^-3 μg·L^-1 in a linear detection range from 0.01 to 1.00μg·L^-1 good recoveries between 105% and 108%, and high precision with intra- and inter-assay coefficients between 7.9% and 14.3%.
基金
Project supported by the National Natural Science Foundation of China （Nos. 90813013, 2U933UU2）.
