摘要
本实验采用荧光分光光度法, 在体内、体外研究了溴氰菊酯对大鼠脑微粒体中7-乙氧基试卤灵O-脱乙基酶(ethoxyresorufin O-deethylase EROD) 和7-苄氧基试卤灵O-脱烷基酶(benzyloxyresorufin O-dealkylatase BROD) 活性的影响, 结果表明: 大鼠经溴氰菊12.5m g. kg- 1. d- 1 通过腹腔注射连续五天后, 溴氰菊酯明显抑制全脑和大脑皮层EROD酶活性, 其抑制率分别是32.0% 、37.0% ; 全脑、大脑皮层及小脑BROD活性明显被抑制, 其抑制率分别是26.7% 、23.8% 和33.3% , 皆具有显著性差异。在体外, 溴氰菊酯浓度在2×10- 8 ～2×10- 4m ol/L范围内对EROD、BROD活性无明显影响;结果提示溴氰菊酯在体内、体外对脑微粒体作用有差异,
The ethoxyresorufin O deethylase(EROD) and benzhloxyresorufin O dealkylatase(BROD) activities in rat brain were determined by flurophotometry under the treatments of deltamethrin(DM) in vivo and in vitro.The results showed that in vivo,DM could markedly inhibit EROD activity in rat brain microsome after rats received DM(12 5mg.kg -1 .d -1 ,ip) for 5 days.The inhibitory rates in whole brain,cerebral cortex were 32 0% and 37 7% respectively.DM also reduced BROD activity in whole brain,cerebral cortex and cerebellum,the rates of inhibition were 26 7%,23 8% and 33 3% respectively.However,in vitro,under the concentration of 2×10 -8 ～2×10 -4 mol/L,there were no obvious changes of EROD and BROD activities in rat brain.So the mechanism by which DM inhibit P450 activity in rat brain microsome is related to the fact that DM intervenes in the synthesis of protein.
出处
《现代预防医学》
CAS
1999年第4期435-436,440,共3页
Modern Preventive Medicine
基金
国家自然科学基金!资助重点项目39430110
