摘要
目的 研究国内不同地区ＨＣＶ包膜基因区变异。方法 对国内不同地区慢性丙型肝炎患者的１３株 ＨＣＶ采用ＲＴ－ＰＣＲ技术扩增ＨＣＶ包膜基因区（Ｅ１、 Ｅ２／ＮＳ１）片段，对其中的１００５ｂｐ的ｃＤＮＡ进行测序，并与 国内外株进行变异性分析。结果 国内ＨＣＶ株比较，同型（Ⅱ）间该区核苷酸和氨基酸的同源性分别介于７６．４２％～ ９７．１１％和７５．２２％～９３．１５％，不同型（Ⅲ）间分别为６２．２９％～６３．３８％和５１，２９％～６０．００％；国内与国际同型株比较，同型 （Ⅱ）间分别为７３．７３％～８７．７６％和７５．８２％～８８．０９５％，不同型（Ⅰ、Ⅱ、Ⅲ）间分别为６１．４９％～８１．４９％和４９．３５％～７８．２１％；国 内外１８株ＨＣＶ的ＨＶＲ１区同源性仅为３６．１１％～７５、３１％和２０．８０％～６２．９６％，发现两个新的较高变异区（第２５０～２５７ 位和第４４３～４６５位氨基酸）。结论 ＨＣＶ包膜区序列分析同型间同源性较高，异型间同源性较低；南北地区有差 异，国内外差异更大；最大的变异在ＨＶＲ１，并发现两个新的较高变异区。
Objective To investigate the variability of E1,E2/NS1 region of hepatitis C virus (HCV) from Chinese isolates. Methods The glycoprotein genes E1 and E2/NS1 of HCV strains from thirteen Chinese patients with chronic hepatitis C from different regions were amplified by RT-PCR.Totally 1005 hp cDNA products were obtained and then sequenced with dideoxynucleotide chain termination method. Nucleotide and amino acid sequences from HCV hypervariable region(HVR1) and flanking regions of the E1 and E2/NS1 were analyzed and compared with previously reported isolates. Results (1) Thirteen HCV isolates of domestic strains were compared. The nucleotide(nt) and amino acid (aa) homology of the same genotype(Ⅱ) were 76.42%-97. 11% and 75.22%-93.15%, respectively. The nt and aa homology between genotype Ⅱ and Ⅲ were 62.29%-63.38% and 51.29%-60.00%, respectively. (2) HCV isolates were compared among strains from China, Japan (HCV-J, HCV-BK, HCV-J6) and America (HCV-1). The homology of the same genotype (Ⅱ) was 73.73%-87.76% and 75.82%-88.09%, respectively. Among the different genotype(Ⅰ,Ⅱ,Ⅲ), the nt and aa homology were 61 .49%81 .49% and 49.35%-78.21 % respectively. (3) The HVR1 at the NH2 terminus of E2 was the most variable portion of the HCV genome. The nt and aa homolongy among thirteen HCV isolates of China,Japan and America strains were only 36. 11 %-75.31% and 20.80%-62.96%. (4) sBesides HVR1 and HVR2 reported previously, two other highly variable regions were found(aa250-257 and 443-465). Conclusion These date suggest that the homology of HCV E1,E2/NS 1 region is related to genotype and the region derived. The HVR1 shows the greatest change in genetic diversity. Two other highly variable regions were found for the first time. This study is beneficial for HCV vaccine design.
出处
《肝脏》
1999年第4期200-204,共5页
Chinese Hepatology
基金
本课题受上海市科委基金资助!(No.984119007)
