摘要
ＭＳ培养基中添加镧（Ｌａ）（０ ．０１ ～０ ．１０ｍ ｍｏｌ·Ｌ－ １） 培养烟草愈伤组织，其总Ｃａ２ ＋ 及原生质体Ｃａ２ ＋ 含量明显比对照（ 不加Ｌａ） 低；长时间（ 继代培养３０ｄ） 较短时间培养（ 悬浮培养２４ｈ） 低．低浓度（０ ．０１ ～０ ．０５１ｍｏｌ·Ｌ－ １）Ｌａ３ ＋ 使细胞壁中Ｃａ２ ＋ 含量高于对照并随浓度提高而增加，高浓度（０ ．１０１ｍｏｌ·Ｌ－ １） 则减少．与Ｃｅ２ ＋ 相比较，Ｌａ３ ＋ 的排Ｃａ２ ＋ 作用稍弱于Ｃｅ３ ＋ ．以不同浓度Ｌａ（ＮＯ３）３ 溶液浸种，油菜幼根总Ｃａ２ ＋ 及原生质体中Ｃａ２ ＋ 含量变化与上述类似．Ｌａ３ ＋ 使烟草愈伤组织褐化明显，生长量比对照低．
The Ca contents in tobacco callus and in its protoplasts were lower in La contained (0.01～0.10mmol·L -1 ) MS medium than in the control (without La addition). As the culture time increased from 1 day to 30 days, the Ca contents declined rapidly. At low La concentration (0.01～0.05mmol·L -1 ), the Ca content in cell wall was higher than that of the control, and increased with increasing La concentration; while at high La concentration (0.10mmol·L -1 ), it decreased. Compared with Ce, La had a weaker effect on Ca exudation. After oilseed rape seeds were soaked with different concentrations of La(NO 3) 3 solution, the dynamics of Ca content in their seedling roots and protoplasts were the same as above mentioned. La made the tobacco callus quickly browned, and the increment of fresh weight was lower than that of the control.
出处
《应用生态学报》
CAS
CSCD
1999年第6期739-742,共4页
Chinese Journal of Applied Ecology
基金
安微省科委"九五"重点项目
