摘要
采用聚合酶链式反应（PCR）和mtDNA细胞色素b基因314hp核音酸序列测定等方法，证明在大石鸡与山石鸡的异域种群之间存在广泛的差异。然而采自六盘山地区与山石鸡相邻分布的大石鸡种群的样本中却存在山石鸡的mtDNA基因型，这种基因型与其它大石鸡个体的基因型有4．4％核着酸差异。在与山石鸡相邻分布的大石鸡种群中有25％的个体具有山石鸡的mtDNA基因型，而采自六盘山接触地带以西约200km处兰州市郊的大石鸡标本中则没有发现这种基因型。此发现支持山石鸡和大石鸡之间存在或曾经有过杂交的假设。作者还提出了一种限制性内切酶分析方法，可以快速地检测这种mtDNA基因型。在此之前还没有过关于山石鸡和大石鸡之间基因流动的报道。
Using polymerase chain reaction (PCR) and direct nucleotide sequencing of the mitochondrial cytochrome b gene, we found an extensive divergence between allopatric samples of Alectoris magna and Alectoris chukar, and the presence of A. chukar haplotype in parapatric samples of A. magna colleced in the Liupanshan (Gansu Province) of China. We have developed a restriction enzyme assay allowing fast identification of these haplotypes, which differ at 4. 4% of their nucleotide sequences. A Population of A. magna sampled in an area of parapatry with A. chukar showed 25% identity of A. chukar mitochondrial haplotype,while the same haplotype was absent in a population of A. magna sampled 2OO km from the Liupanshan contact zone. These findings support the hyPothesis of actual or historical hybridization between A. chukar and A. magna, with consequent mitochondria1 DNA introgres-sion. Hybridization and gene flow between A. chukar and A. nzagna had not been documentedbefore these molecular genetic findings.
出处
《动物学报》
SCIE
CAS
CSCD
1999年第4期456-463,共8页
ACTA ZOOLOGICA SINICA
关键词
线粒体DNA
杂交
山石鸡
大石鸡
邻城分布
Mitochondrial DNA, Hybridization, Alectoris magna, Alectoris chukar
