摘要
研究了不同碳氮源对金龟子绿僵菌（ Ｍｅｔａｒｈｉｚｉｕｍ ａｎｉｓｏｐｌｉａｅ） 产生与分解昆虫外壳相关的蛋白酶活性和同工酶谱带的影响． 结果表明：蝉蜕、虻虫壳、蝇蛆壳、蚕蛹壳、虾壳、胶体几丁质均可使金龟子绿僵菌产生蛋白酶，虾壳和胶体几丁质所产生的蛋白酶比活性最高，蝉蜕次之． 但蛋白酶总活性以蝉蜕最高，虻虫壳次之．样品经等电聚焦电泳后，印迹于Ｘ光片上，显示蛋白酶同工酶，蝉蜕和蚕蛹壳产生的蛋白酶同工酶谱带最丰富． 以蝉蜕为唯一碳氮源时，金龟子绿僵菌产生蛋白酶的最佳条件为２６℃、初始ｐＨ６．０ 、最终孢子浓度ｎ＝ １×１０７ｍＬ－１ ．
Several inducers cicada exuviae, colloidal chitin, horsefly cuticle, shrimp, maggot and silkworm chrysalis were compared with their effects on production of cuticle-degrading protease of entomopathogenic fungus Metarhizium anisopliae . The results indicated that the highest specific activity could be produced by colloidal chitin and shrimp cuticle, and then by cicada exuviae. The optimum cultivation conditions were 26℃, initial pH 6.0 and inoculum quantity of spore suspension n =1×10 7mL -1 , using cicada exuviae as the inducer.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第6期601-604,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金!(No:39570022)
