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三株产甲烷细菌表面层研究 被引量:1

STUDY ON THE SURFACE LAYERS OF THREE METHANOGENS
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摘要 用电子显微镜的负染色技术,对史氏甲烷短杆菌H13( Methanobrevibactersmithii H13)、嗜热甲酸甲烷杆菌CB12( Methanobacterium thermoformicicum CB12) 、拉布雷甲烷粒菌Z( Methanocorpusculum labreanum Z) 的表面结构观察比较结果表明:史氏甲烷短杆菌H13 和嗜热甲酸甲烷杆菌CB12 的表面层由规则排列的四边形亚单位组成;前者每个亚单位直径为5~7 nm ,亚单位之间的中心距离为5 ~8 nm ,后者亚单位直径为9~12 nm ,亚单位之间的中心距离为11~14 nm .拉布雷甲烷粒菌Z的表面层是条纹和颗粒状或丝状组成的网状结构.每根条纹宽3~4 nm ,条纹之间的距离为6~8 nm .3 株产甲烷细菌的表面层各不相同,其中嗜热甲酸甲烷杆菌CB12 和拉布雷甲烷粒菌Z的表面层结构十分独特,明显不同于已报道的产甲烷细菌和古细菌的表面层结构. The surface layers of three strains of methanogens,including Methanobrevibacter smithii H13, Methanobacterium thermoformicicum CB12 and Methanocorpusculum labreanum Z,were examined by negative staining preparation with electron microscope.Two types of crystallin protein array could be observed in the three methanogens.The surface layers of Methanobrevibacter smithii H13 and Methanobacterium thermoformicicum CB12 were tetragonally arranged by protein subunits.The diameter of each subunit of the former was 5~7 nm,and center to center space of subunits was 9~12 nm,and those of the latter were 9~12 nm and 11~14 nm,respectively.The surface layer of Methanocorpusculum labreanum Z was reticulate and consisted of a series of striae and irregular granules.Each stria was 3~4 nm wide and the distance between striae was 6~8 nm.This study showed that the surface layers of the three methanogens were different and also different from those of the reported methanogens and archaebacteria.
作者 郑中华 张辉
出处 《应用与环境生物学报》 CAS CSCD 1999年第6期610-613,共4页 Chinese Journal of Applied and Environmental Biology
基金 中国科学院留学经费择优支持项目 中国科学院成都地奥科学基金
关键词 表面层 形态结构 产甲烷细菌 古细菌 H13 CB12 Methanobrevibacter Smithii H13 Methanobacterium thermoformicicum CB12 Methanocorpusculum labreanum Z surface layer morphological structure
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  • 1Chen H M,Protein Eng,1995年,8卷,7期,575页

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