摘要
采用酸性染料比色法测定红茂草中总生物碱含量。以异紫定堇碱为标准品,酸性染料比色法的显色条件为:pH值4.0、波长415 nm、溴甲酚绿1.5 mL,得到标准曲线:y=0.032 x-0.033,R2=0.977 0,生物碱检测浓度在21.6-54.0 mg/mL范围内与吸收度线性关系良好,红茂草中总生物碱含量为1.386%。该方法灵敏度高、准确度好、重现性好。
The content of total alkaloid in Dicranostigma leptodum（Maxim.） Fedde（DLF） was determined by acid dye colorimetry.Using the isocorydine as standard,acid dye colorimetry conditions were as follows： pH=4.0,wavelength of 425 nm,bromocresol green liquid 1.5 mL.The standard curve was then obtained： y=0.032 x-0.033,R2=0.977 0.The concentration of alkaloid showed good linearity with its absorbability within the range of 21.6-54.0 mg/mL.The content of total alkaloid from DLF was 1.386%.The method is simple,highly sensitive and reproducible.
出处
《中兽医医药杂志》
2011年第6期36-38,共3页
Journal of Traditional Chinese Veterinary Medicine
基金
甘肃省中医药管理局基金资助项目(GZK-2010-20)
关键词
酸性染料比色法
红茂草
总生物碱
acid dye colorimetry
Dicranostigma leptodum（Maxim.） Fedde
total alkaloid
