摘要
采用大孔树脂对藿香蓟花蓝色素的提取液进行了吸附和洗脱试验,并确定藿香蓟花蓝色素的分离最佳工艺条件。结果表明,D301树脂对藿香蓟花蓝色素的静态吸附率达到90mg/g,吸附蓝色素的平衡时间较短且重复使用20次后吸附性能依然稳定;上柱优化条件为流速为3mL/min,质量浓度为0.2mg/mL,温度为20℃;柱层析饱和吸附后用1mol/L盐酸洗脱,洗脱率达到96.08%。
Absorption on macroporous resin was studied as a method to separate Ageratum conyzoides Flowers blue pigment in this manuscript.The optimum conditions for absorption and desorption of Ageratum conyzoides Flowers blue pigment was determined and listed as follows： D301 with the upper adsorption rate at 90.0mg/g,the stability and the little balance time was suitable for absorbing and separating Ageratum conyzoides Flowers blue pigment.The optimum condition was at the flow rate of 2.0mL/min,concentration of 0.2 mg/mL and the temperature of 20 ℃.D301 was washed with HCl of 2.0mol/L,and the washout rate achieved at 96.08%.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2011年第6期852-856,共5页
Journal of Food Science and Biotechnology
基金
山东省自然科学基金项目(2009GG10007014)
关键词
藿香蓟
蓝色素
吸附分离
大孔树脂
Ageratum conyzoides
blue pigment
adsorption and separation
resin
