摘要
目的寻求一种简便有效的早孕小鼠子宫内膜基质细胞的培养方法。方法采用胰蛋白酶消化与机械振荡相结合分离小鼠子宫内膜基质细胞,接种后通过光镜观察其形态学特征,并采用免疫细胞化学进行细胞鉴定。结果接种24h后大部分细胞已贴壁,培养3d后细胞排列紧密、形态为长梭形。经过免疫细胞化学鉴定,成功获得了小鼠子宫内膜基质细胞。结论胰蛋白酶消化与机械振荡相结合可成功分离并原代培养小鼠子宫内膜基质细胞。
Objective To seek a simple and effective way to culture endometrial stromal cells from mice in early pregnancy.Methods Stromal cells from mouse endometrium were isolated by combination of trypsin digestion and mechanical vibration.Their morphological characteristic were observed by optical microscopy after seeding,and the cellular identification was performed using immunocytochemistry.Results Most cells became adherent 24 hours after seeding,and they were tightly packed with long spindle-shaped morphology after 3 days of culture.Mouse endometrial stromal cells were successfully cultivated which was indentified by immunocytochemistry.Conclusion Mouse endometrial stromal cells can be successfully isolated and primary cultured by means of combination of trypsin digestion and mechanical vibration.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第34期3472-3473,F0003,共3页
Chongqing medicine
