摘要
目的建立复方金钱草排石颗粒中槲皮素和山柰素的含量测定方法。方法采用高效液相色谱法,C18色谱柱(250 mm×4.6 mm),以甲醇-0.4%磷酸溶液(50∶50)为流动相,检测波长为360 nm,流速为1.0 ml·min-1。结果槲皮素线性范围为0.002 7～0.107 5μg,平均回收率=101.13%,RSD=2.40%;山柰素线性范围为0.012 3～0.491 2μg,平均回收率=98.66%,RSD=1.22%。结论该法准确、简便、快捷,可以同时测定复方金钱草排石颗粒中槲皮素和山柰素的含量。
Aim To establish a method for determination of quercetin and kaempferide in Fufang Jinqiancao Paishi Keli.Methods By high performance liquid chromatography（HPLC） method,the C18 column（250 mm×4.6 mm）was adopted.The mobile phase consisted of methanol-0.4% phosphoric acid（50∶ 50）,the detection wavelength was 360 nm and the flow rate was 1.0 ml·min^-1.Results The linear range of quercetin was 0.002 7-0.107 5 μg.The average recovery rate was 101.13%,and RSD was 2.40%.The linear range of kaempferide was 0.012 3~0.491 2 μg.The average recovery rate was 98.66%,and RSD was 1.22%.Conclusion This method is accurate,simple and quick.It can assay quercetin and kaempferide in Fufang Jinqiancao Paishi Keli simultaneously.
出处
《安徽医药》
CAS
2011年第12期1510-1512,共3页
Anhui Medical and Pharmaceutical Journal
关键词
复方金钱草排石颗粒
高效液相色谱法
槲皮素
山柰素
Fufang Jinqiancao Paishi Keli
high performance liquid chromatography method
quercetin
kaempferide
